CAJ | 학술논문

目的：探讨卤米松、黄芩提取物联合用药对莫诺苯宗( monobenzone)诱导的白癜风小鼠模型发生发展的影响。方法应用40%莫诺苯宗乳膏诱导C57BL/6小鼠脱色,建立白癜风小鼠模型；研究卤米松( halometasone)联合黄芩提取物( scutellaria baicalensis georgi)用药对白癜风小鼠的脱色作用；通过肉眼观察小鼠的毛发脱色,反射共聚焦显微镜( re-flectance confocal microscopy, RCM)观察皮肤的黑色素和黑素细胞,免疫荧光检测CD8+T细胞,酶联免疫吸附剂测定( enzyme linked immunosorbent assay,ELISA)测定血清中白介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor,TNF-α)的含量。结果模型组小鼠在用药部位及非用药部位有毛发脱色现象。黄芩组疗效不明显,卤米松组和卤米松联合黄芩组小鼠脱色减少,包括其发生率、出现时间和面积评分较模型组低；且局部CD8+T细胞浸润减少；卤米松联合黄芩组小鼠血清中IL-6、TNF-α的含量减少明显。结论卤米松、黄芩提取物联合用药对白癜风小鼠有一定的作用,且效果优于单独使用卤米松组和黄芩组,可为临床白癜风的治疗提供参考。
목적：탐토서미송、황금제취물연합용약대막낙분종( monobenzone)유도적백전풍소서모형발생발전적영향。방법응용40%막낙분종유고유도C57BL/6소서탈색,건립백전풍소서모형；연구서미송( halometasone)연합황금제취물( scutellaria baicalensis georgi)용약대백전풍소서적탈색작용；통과육안관찰소서적모발탈색,반사공취초현미경( re-flectance confocal microscopy, RCM)관찰피부적흑색소화흑소세포,면역형광검측CD8+T세포,매련면역흡부제측정( enzyme linked immunosorbent assay,ELISA)측정혈청중백개소-6(interleukin-6,IL-6)、종류배사인자-α(tumor necrosis factor,TNF-α)적함량。결과모형조소서재용약부위급비용약부위유모발탈색현상。황금조료효불명현,서미송조화서미송연합황금조소서탈색감소,포괄기발생솔、출현시간화면적평분교모형조저；차국부CD8+T세포침윤감소；서미송연합황금조소서혈청중IL-6、TNF-α적함량감소명현。결론서미송、황금제취물연합용약대백전풍소서유일정적작용,차효과우우단독사용서미송조화황금조,가위림상백전풍적치료제공삼고。
Aim To study the effect of halometasone in combination with scutellaria baicalensis georgi on the vitiligo mice induced by monobenzone. Methods 40% monobenzone cream was applied to induce vitiligo in C57BL/6 mice. Through the halometasone, halo-metasone and scutellaria baicalensis georgi combined with 40% monobenzone cream, the influence of halo-metasone and scutellaria baicalensis georgi on mice de-colorizing was studied. Hair decolorizing was observed with the naked eye, the skin decolorizing was observed by reflectance confocal microscopy ( RCM ) , and CD8 +T cell infiltration was tested with immunofluores-cence detection. The serum levels of interleukin-6(IL-6 ) and tumor necrosis factor-α( TNF-α) were deter-mined by enzyme linked immunosorbent assay ( ELISA) . Results Mice in model group showed de-pigmentation at both the monobenzone application part and non-application part. The halometasone group did not show significant therapeutic efficacy. In halometa-sone and scutellaria baicalensis georgi treatment group, there was less decolorization, the occurrence ratio, the scores of occurring time and size were lower compared with model group. There were fewer infiltrated lympho-cytes and CD8 +T cells. Halometasone and scutellaria baicalensis georgi group also showed that the serum levels of IL-6,TNF-α decreased. Conclusion Halo-metasone and scutellaria baicalensis georgi have thera-peutic effect on vitiligo mice induced by monobenzone.