CAJ | 학술논문

目的：探讨实验性应激对糖尿病大鼠血糖、胰岛素、胰高糖素、皮质醇、肾上腺素水平和胰岛素抵抗指数的影响以及胰岛素和氟西汀对此的干预作用。方法实验大鼠分为正常大鼠对照组、糖尿病大鼠对照组、糖尿病大鼠胰岛素组、糖尿病大鼠氟西汀组，高糖高脂饲养加腹腔少量注射链脲菌素诱导糖尿病大鼠模型，各组大鼠进行为期8周的随机强迫游泳、寒冷、旋转、限制、拥挤等实验性应激，实验第4、8周分别采血测定血清血糖、胰岛素、胰高糖素、皮质醇、肾上腺素水平。结果糖尿病大鼠应激后血糖[（16.7±3.5）mmol／L vs （5.1±1.1）mmol／L， t ＝13.9， P ＜0.01]、胰高糖素[（158.5±50.2）ng／L vs （120.8±38.7）ng／L， t ＝2.5， P ＜0.05]、肾上腺素[（203.8±48.6）pg／ml vs （158.7±42.6）pg／ml， t ＝2.9， P ＜0.01]、皮质醇水平[（21.3±4.8）ng／ml vs （18.2±3.8）ng／ml， t ＝2.1， P ＜0.05]及胰岛素抵抗指数（10.9±2.6 vs 3.3±0.8， t ＝12.3， P ＜0.01）明显升高，胰岛素治疗后血糖[（9.7±2.1）mmol／L vs （16.7±3.5）mmol／L， t ＝7.0， P ＜0.01]明显改善，氟西汀治疗后肾上腺素[（158.8±37.5）pg／ml vs （203.8±48.6）pg／ml， t ＝3.0， P ＜0.01]、皮质醇[（15.7±4.2）ng／ml vs （21.3±4.8）ng／ml， t ＝3.5， P ＜0.01]明显改善。结论实验性应激导致糖尿病大鼠血糖和内分泌激素升高，加重胰岛素抵抗，胰岛素能改善应激后血糖变化，氟西汀能改善应激后内分泌激素的升高。
목적：탐토실험성응격대당뇨병대서혈당、이도소、이고당소、피질순、신상선소수평화이도소저항지수적영향이급이도소화불서정대차적간예작용。방법실험대서분위정상대서대조조、당뇨병대서대조조、당뇨병대서이도소조、당뇨병대서불서정조，고당고지사양가복강소량주사련뇨균소유도당뇨병대서모형，각조대서진행위기8주적수궤강박유영、한랭、선전、한제、옹제등실험성응격，실험제4、8주분별채혈측정혈청혈당、이도소、이고당소、피질순、신상선소수평。결과당뇨병대서응격후혈당[（16.7±3.5）mmol／L vs （5.1±1.1）mmol／L， t ＝13.9， P ＜0.01]、이고당소[（158.5±50.2）ng／L vs （120.8±38.7）ng／L， t ＝2.5， P ＜0.05]、신상선소[（203.8±48.6）pg／ml vs （158.7±42.6）pg／ml， t ＝2.9， P ＜0.01]、피질순수평[（21.3±4.8）ng／ml vs （18.2±3.8）ng／ml， t ＝2.1， P ＜0.05]급이도소저항지수（10.9±2.6 vs 3.3±0.8， t ＝12.3， P ＜0.01）명현승고，이도소치료후혈당[（9.7±2.1）mmol／L vs （16.7±3.5）mmol／L， t ＝7.0， P ＜0.01]명현개선，불서정치료후신상선소[（158.8±37.5）pg／ml vs （203.8±48.6）pg／ml， t ＝3.0， P ＜0.01]、피질순[（15.7±4.2）ng／ml vs （21.3±4.8）ng／ml， t ＝3.5， P ＜0.01]명현개선。결론실험성응격도치당뇨병대서혈당화내분비격소승고，가중이도소저항，이도소능개선응격후혈당변화，불서정능개선응격후내분비격소적승고。
Objective To investigate the impact of experimental stress on serum glucose , insulin, glucagon, cortisol, and ep-inephrine in diabetic rats and its intervention with insulin and fluoxetine .Methods Type 2 diabetic rats model was induced by feeding with high sugar and high fat diet , and intraperitoneal injection of streptozotocin (STZ).The rats were divided into normal group , dia-betic control group , diabetic treated with insulin , and diabetic treated with fluoxetine .All the rats were exposed to multiple stressors (forced swimming, cold stimulation, rotation, restrain, and crowding) for 8 weeks.The rat blood sera collected from each group were analyzed with multiple parameters including glucose , insulin, glucagon, cortisol, and epinephrine at the 4th and 8th week.Results After experimental stress, the levels of glucose [(16.7 ±3.5)mmol/L vs (5.1 ±1.1)mmol/L, t =13.9, P <0.01], glucagon [(158.5 ±50.2)ng/L vs (120.8 ±38.7)ng/L, t =2.5, P <0.05], epinephrine [(203.8 ±48.6)pg/ml vs (158.7 ±42.6)pg/ml, t =2.9, P <0.01], cortisol [(21.3 ±4.8)ng/ml vs (18.2 ±3.8)ng/ml, t =2.1, P <0.05], and HOMA-IR (10.9 ±2.6 vs 3.3 ±0.8 , t =12.3 , P <0.01 ) of diabetic rats were significantly increased .The glucose level [ ( 9.7 ±2.1 ) mmol/L vs ( 16.7 ± 3.5)mmol/L, t =7.0, P <0.01] was significantly improved after treated with insulin .The levels of epinephrine [(158.8 ±37.5 ) pg/ml vs (203.8 ±48.6)pg/ml, t =3.0, P <0.01], and cortisol [(15.7 ±4.2)ng/ml vs (21.3 ±4.8)ng/ml, t =3.5, P <0.01 ] were significantly improved after treated with fluoxetine .Conclusions Experimental stress increased the levels of glucose , en-docrine hormone , and insulin resistance of diabetic rats .Treatment with insulin improved the glucose level and treatment of fluoxetine improved the endocrine hormone level .