CAJ | 학술논문

目的：观察不同浓度尿酸对人肾小管上皮细胞（HK-2）相关纤维化调控因子的影响，探讨建立尿酸诱导的人肾小管上皮细胞纤维化模型，为尿酸性肾纤维化疾病的研究提供细胞模型。方法以不同浓度的尿酸刺激HK-2细胞6，12，24，36 h ，MTT检测细胞活性抑制率；Real-Time PCR检测TGF-β1、CTGF、α-SMA mRNA的表达，观察尿酸对 HK-2细胞的促纤维化作用；26 mg/dL浓度尿酸刺激HK-2细胞24 h ，免疫组织化学检测TGF-β1、CTGF、α-SMA蛋白的改变。结果尿酸刺激后细胞活性抑制率与对照组比较明显升高（P＜0．05），呈时间依赖性；尿酸刺激后TGF-β1、CTGF、α-SMA的表达量与对照组比较明显增加（P＜0．05），并呈剂量依赖性；尿酸刺激后TGF-β1、CTGF、α-SMA蛋白的表达量与对照组比较明显增加（P＜0．05）。结论尿酸可抑制 HK-2细胞增殖；采用尿酸诱导 HK-2细胞，可建立肾纤维化的细胞模型。
목적：관찰불동농도뇨산대인신소관상피세포（HK-2）상관섬유화조공인자적영향，탐토건립뇨산유도적인신소관상피세포섬유화모형，위뇨산성신섬유화질병적연구제공세포모형。방법이불동농도적뇨산자격HK-2세포6，12，24，36 h ，MTT검측세포활성억제솔；Real-Time PCR검측TGF-β1、CTGF、α-SMA mRNA적표체，관찰뇨산대 HK-2세포적촉섬유화작용；26 mg/dL농도뇨산자격HK-2세포24 h ，면역조직화학검측TGF-β1、CTGF、α-SMA단백적개변。결과뇨산자격후세포활성억제솔여대조조비교명현승고（P＜0．05），정시간의뢰성；뇨산자격후TGF-β1、CTGF、α-SMA적표체량여대조조비교명현증가（P＜0．05），병정제량의뢰성；뇨산자격후TGF-β1、CTGF、α-SMA단백적표체량여대조조비교명현증가（P＜0．05）。결론뇨산가억제 HK-2세포증식；채용뇨산유도 HK-2세포，가건립신섬유화적세포모형。
Objective To observe the effect of different concentrations of uric acid (UA) on ex-pression of fibrosis-related regulatory factors in human renal tubular epithelial cells (HK-2) ,and to estab-lish UA-induced fibrosis model in HK-2 cells for study of renal fibrosis diseases . Methods HK-2 cells were exposed to increasing concentrations of UA for 6h,12h,24h,36h,respectively ,and the cell via-bility was determined by MTT assay . Real-Time PCR were employed to investigate the mRNA expres-sion of TGF-β1 ,CTGF ,andα-SMA . The protein levels of TGF-β1 ,CTGF ,and α-SMA were evaluated by immunohistochemistry on HK-2 cells exposed to UA for 24h. Results MTT assay showed that UA could induce marked decrease of cell viability in a time-dependent manner as compared with the untreated control cells(P<0 .05) . The mRNA levels of TGF-β1 ,CTGF and α-SMA were increased time-depend-ently when HK-2 cells were treated with with UA (P<0 .05) . The protein levels of TGF-β1 ,CTGF andα-SMA were increased significantly in HK-2 cells treated with 26 mg/dL UA for 24 h(P<0 .05) . Conclu-sion UA could inhibit HK-2 cell proliferation and the cells model of renal fibrosis could be established by UA induction .