CAJ | 학술논문

以丙酮丁醇梭菌为发酵用菌株发酵水蒸气爆破玉米秸秆酶解上清液生产丁醇，研究了酶解上清液浓度、初始pH值以及菌液接种量对丁醇产量的影响，并通过正交试验来确定营养元素的最佳添加量．结果表明：在丙酮丁醇梭菌发酵水蒸气爆破玉米秸秆酶解上清液生产丁醇的最佳条件下（上清液糖浓度57．5 g/L、初始pH值6.3，菌液接种量6％，发酵温度37℃，营养元素酵母膏、乙酸铵、磷酸二氢钾、烟酰胺添加量分别为0.8、6.0、0.5、0.25 g/L），丁醇产量达9.726 g/L；水蒸气爆破玉米秸秆酶解液中有抑制产生溶剂的物质，少量烟酰胺能有效促进丁醇生产．
이병동정순사균위발효용균주발효수증기폭파옥미갈간매해상청액생산정순，연구료매해상청액농도、초시pH치이급균액접충량대정순산량적영향，병통과정교시험래학정영양원소적최가첨가량．결과표명：재병동정순사균발효수증기폭파옥미갈간매해상청액생산정순적최가조건하（상청액당농도57．5 g/L、초시pH치6.3，균액접충량6％，발효온도37℃，영양원소효모고、을산안、린산이경갑、연선알첨가량분별위0.8、6.0、0.5、0.25 g/L），정순산량체9.726 g/L；수증기폭파옥미갈간매해액중유억제산생용제적물질，소량연선알능유효촉진정순생산．
In this paper,butanol was prepared from the enzymatic hydrolysate supermatant of steam-exploded corn stover,with Clostridium acetobutylicum as the experimental bacterium.Then,the effects of supermatant concentra-tion,initial pH value and microbial inoculation quantity on butanol yield were investigated,and the optimal dosages of nutrient elements were determined by orthogonal test.The results show that,under optimal conditions,that is,a supermatant sugar content of 57.5 g/L,an initial pH value of 6.3,an inoculation quantity of 6%,a fermentation temperature of 37 ℃,and a nutrient elements addition of yeast extract 0.8 g/L+CH3 COONH4 6.0 g/L+KH2 PO4 0.5 g/L+nicotinamide 0.25 g/L,the butanol yield is up to 9.726 g/L.Moreover,it is also found that there exist some inhibitors for the solvent production from steam-exploded corn stover enzymatic hydrolysate,and that a small amount of nicotinamide may effectively promote butanol production.