CAJ | 학술논문

目的：观察燃煤型砷中毒大鼠肾毒性作用，应用基准剂量（BMD）法探讨肾功能效应标志改变的尿砷（UAs）的基准剂量。方法Wistar 大鼠连续90 d 喂饲砷25，50，100 mg·kg -1污染饲料。检测大鼠UAs、肾砷（KAs）及肾功能指标并观察肾常规病理学及其纤维化改变。以 UAs 为暴露标志，尿β2-微球蛋白微球蛋白（Uβ2-MG）、尿 N-乙酰-β-D-氨基葡萄糖苷酶（UNAG）及尿白蛋白（UALB）为效应标志，运用 BMD法计算各效应标志对应的 UAs 的 BMD 及其基准剂量95％可信限下限 BMDL。结果砷100 mg·kg -1组大鼠 UAs，KAs，UALB，UNAG，Uβ2-MG 含量均高于正常对照组（P ＜0．05）；光镜下各染砷组大鼠肾 HE染色可见炎性细胞浸润、肾小管上皮细胞肿胀、肾间质毛细管扩张、充血等不同程度的病理学改变，Masson染色可见肾小管间质不同程度的纤维化；以 UAs 为暴露标志，Uβ2-MG，UNAG，UALB 为效应标志，计算得出各效应标志 UAs 的 BMD 分别为998．9，1213．5和1386．9μg·g -1 Cr，BMDL 分别为660．5，803．6和909．4μg·g -1 Cr。燃煤型砷中毒大鼠肾功能早期改变的尿砷的 BMD 及 BMDL 分别为998．9和660．5μg·g -1 Cr。结论微小病变型肾病可能是燃煤砷污染对肾损害的主要病理类型，推荐使用较为敏感的生物学标志Uβ2-MG来估算砷致肾损伤的生物暴露限值。
목적：관찰연매형신중독대서신독성작용，응용기준제량（BMD）법탐토신공능효응표지개변적뇨신（UAs）적기준제량。방법Wistar 대서련속90 d 위사신25，50，100 mg·kg -1오염사료。검측대서UAs、신신（KAs）급신공능지표병관찰신상규병이학급기섬유화개변。이 UAs 위폭로표지，뇨β2-미구단백미구단백（Uβ2-MG）、뇨 N-을선-β-D-안기포도당감매（UNAG）급뇨백단백（UALB）위효응표지，운용 BMD법계산각효응표지대응적 UAs 적 BMD 급기기준제량95％가신한하한 BMDL。결과신100 mg·kg -1조대서 UAs，KAs，UALB，UNAG，Uβ2-MG 함량균고우정상대조조（P ＜0．05）；광경하각염신조대서신 HE염색가견염성세포침윤、신소관상피세포종창、신간질모세관확장、충혈등불동정도적병이학개변，Masson염색가견신소관간질불동정도적섬유화；이 UAs 위폭로표지，Uβ2-MG，UNAG，UALB 위효응표지，계산득출각효응표지 UAs 적 BMD 분별위998．9，1213．5화1386．9μg·g -1 Cr，BMDL 분별위660．5，803．6화909．4μg·g -1 Cr。연매형신중독대서신공능조기개변적뇨신적 BMD 급 BMDL 분별위998．9화660．5μg·g -1 Cr。결론미소병변형신병가능시연매신오염대신손해적주요병리류형，추천사용교위민감적생물학표지Uβ2-MG래고산신치신손상적생물폭로한치。
OBJECTIVE Study the kidney toxic effects caused by burning coal endemic arsenism in rats,application bench mark dose (BMD) method to investigate the bench mark dose of urinary arsenic (UAs)and the changes in bio markers of renal function.METHODS Wistar rats were fed for 90 d with arsenic 0,25,50,100 mg·kg -1 conta minated feed.Urinary arsenic,kidney arsenic and renal function indicators were determined,and routine pathological and fibrosis of kidney were exa mined.UAs as the exposure bio marker,Uβ2-MG,UNAG and UALB for the effect bio markers,application bench mark dose method to calculate the BMD and BMDL of UAs for each effect bio markers.RESULTS UAs,KAs, Uβ2-MG,UNAG,UALB levels of rats in arsenic 100 mg·kg -1 group were increased than normal group (P <0.05);In light microscope,the results of HE staining of rat kidney in all arsenic dose groups showed infla mmatory cell infiltration,renal tubular epithelial cell swelling,renal interstitial capillary dila-tion,congestion and other varying degrees pathological changes,and the results of masson staining showed varying degrees of tubulointerstitial fibrosis;UAs as the exposure bio marker,Uβ2-MG,UNAG, UALB for the effects of mark,the BMD and BMDL of UAs for Uβ2-MG,UNAG,UALB were calculated, the BMD values were 998.9,1213.5,1386.9 μg·g -1 Cr,the BMDL values were 660.5,803.6 and 909. 4 μg·g -1 Cr,respectively.CONCLUSION Burning coal arsenic pollution can cause kidney da mage in rats,mini mal change nephropathy may be the pri mary pathological in the coal arsenic conta mination of kidney da mage.The BMD and BMDL of UAs were 998.9,660.5 μg·g -1 Cr,the early changes of renal function of burning coal arsenism in rats;it is reco mmended to use the more sensitive bio markers Uβ2-MG to calculate the biological exposure li mits on renal injury caused by arsenic.