CAJ | 학술논문

目的：探讨有氧运动对有机磷农药马拉硫磷染毒大鼠肠神经毒效应的影响。方法成年雄性Wistar 大鼠进行无负重游泳，隔天1次，每周3次，每次1 h，持续6周；运动前 ig 给予马拉硫磷染毒100 mg·kg -1·d -1，每周6 d，持续6周。测定血清乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）活性，测定小肠推进指数；观察回肠神经丛分布，免疫组化法测定回肠蛋白基因相关肽9．5（PGP9．5）、P 物质（SP）和血管活性肠肽（VIP）的表达水平。结果与正常对照组相比，马拉硫磷染毒组大鼠 AChE 和BuChE 活性显著下降（P ＜0．01），小肠推进指数显著升高，回肠 PGP9．5水平显著下降，SP 水平显著升高，VIP 水平显著下降（P＜0．05）；有氧运动组胆碱酯酶活性无变化，小肠推进指数显著降低，回肠 PGP9．5水平显著升高，SP 水平显著降低，VIP 水平显著升高。与单纯马拉硫磷染毒组相比，染毒运动组大鼠胆碱酯酶活性显著下降（P＜0．01），小肠推进指数由染毒组（89±4）％降至（79±5）％（P ＜0．01），回肠 PGP9．5水平由染毒组0．012±0．003升至0．029±0．015（P ＜0．01），SP 水平由染毒组0．174±0．067降至0．110±0．057（P＜0．05），VIP 水平由染毒组0．0076±0．0029升至0．0111±0．0047（P ＜0．05），恢复至或接近正常对照组水平。结论马拉硫磷染毒干扰大鼠肠神经系统的功能，有氧运动改善马拉硫磷染毒对大鼠的肠神经毒效应，但不改善染毒对血清胆碱酯酶活性的抑制。
목적：탐토유양운동대유궤린농약마랍류린염독대서장신경독효응적영향。방법성년웅성Wistar 대서진행무부중유영，격천1차，매주3차，매차1 h，지속6주；운동전 ig 급여마랍류린염독100 mg·kg -1·d -1，매주6 d，지속6주。측정혈청을선담감지매（AChE）화정선담감지매（BuChE）활성，측정소장추진지수；관찰회장신경총분포，면역조화법측정회장단백기인상관태9．5（PGP9．5）、P 물질（SP）화혈관활성장태（VIP）적표체수평。결과여정상대조조상비，마랍류린염독조대서 AChE 화BuChE 활성현저하강（P ＜0．01），소장추진지수현저승고，회장 PGP9．5수평현저하강，SP 수평현저승고，VIP 수평현저하강（P＜0．05）；유양운동조담감지매활성무변화，소장추진지수현저강저，회장 PGP9．5수평현저승고，SP 수평현저강저，VIP 수평현저승고。여단순마랍류린염독조상비，염독운동조대서담감지매활성현저하강（P＜0．01），소장추진지수유염독조（89±4）％강지（79±5）％（P ＜0．01），회장 PGP9．5수평유염독조0．012±0．003승지0．029±0．015（P ＜0．01），SP 수평유염독조0．174±0．067강지0．110±0．057（P＜0．05），VIP 수평유염독조0．0076±0．0029승지0．0111±0．0047（P ＜0．05），회복지혹접근정상대조조수평。결론마랍류린염독간우대서장신경계통적공능，유양운동개선마랍류린염독대대서적장신경독효응，단불개선염독대혈청담감지매활성적억제。
OBJECTIVE To investigate the effects of aerobic exercise on enteric nervous injury in rats exposed to malathion.METHODS Adult male Wistar rats were treated with non-load swi mming every other day,three ti mes a week,each one hour,for six weeks.Before exercise,the rats were trea-ted with malathion 100 mg·kg -1·d -1 by oral gavage,six days a week,for six weeks.The activities of seru m acetylcholinesterase(AChE)and butyrocholinesterase(BuChE)were determined.In addition,the s mall intestinal propulsion indexes were measured.Also,the distribution of nerve plexus in ileu m was observed.The i mmunohistoche mical method was used to measure the levels of protein gene-related petide 9.5 (PGP9.5),substance P (SP),and vasoactive intestinal peptide (VIP).RESULTS Co m-pared with normal control,malathion exposure decreased the activities of seru m AChE and BuChE (P<0.01 ),increased the s mall intestinal propulsion indexes (P <0.05).In addition,the levels of PGP9.5 decreased (P<0.05).At the sa me ti me,the levels of SP increased,and the levels of VIP decreased (P<0.05).Aerobic exercise did not change the activites of cholinesterases,but decreased s mall intes-tinal propulsion indexes,increased the levels of PGP9.5,decreased the levels of SP,and increased the levels of VIP.Co mpared with the malathion exposure only,the rats in malathion ad ministration co mbined with aerobic exercise group de monstrated much lower activites of cholinesterase (P <0.01 ),and the s mall intestinal propulsion indexes decreased fro m (89 ±4)% to (79 ±5)%(P <0.01 ).Moreover,the levels of PGP9.5 increased fro m 0.012 ±0.003 to 0.029 ±0.015 (P <0.01 ).At the sa me ti me,the levels of SP decreased fro m0.174 ±0.067 to 0.1 10 ±0.057(P<0.05),and the levels of VIP increased fro m 0.0076 ±0.0029 to 0.01 1 1 ±0.0047 (P <0.05).The levels of above para meters were sa me or close to those of the normal control.CONCLUSION Malathion exposure induced disorders of enteric nervous syste m in rats,and the aerobic exercise abated the toxic response in enteric nervous syste m of malathion exposure rats.However,these effects were not mediated through recovery of cholinesterases inhibition.