CAJ | 학술논문

目的：观察维生素 C 对纳米二氧化钛染毒小鼠心脏损伤的保护作用。方法昆明小鼠 ig 给予维生素 C 100，200和400 mg·kg -12 d，后3 d 间隔4 h 后 ig 给予纳米二氧化钛2 g·kg -1，末次染毒后24 h进行指标检测。生理记录仪测定心电图；生化法测定血清中心肌酶活性及血清和心肌组织超氧化物岐化酶（SOD）和谷胱苷肽过氧化酶（GSH-Px）活性；彗星实验检查心肌单细胞 DNA 损伤；HE 染色光镜观察心脏组织病理改变。结果纳米二氧化钛2 g·kg -1染毒小鼠后，心电图检查发现 ST 段抬高、T 波高尖，与正常对照组比差异有显著性（P ＜0．05）；染毒组血清中心肌酶活性均较正常对照组明显升高（P＜0．05），血清和心肌组织中 SOD 和 GSH-Px 活性降低（P＜0．05）。彗星实验结果表明，纳米二氧化钛2 g·kg -1染毒组彗星 Olive 尾距（OTM）显著高于正常对照组（P＜0．05）。组织病理学结果显示，纳米二氧化钛2 g·kg -1染毒后小鼠心肌组织出现细胞水肿、心肌纤维紊乱、炎性细胞增多。给予维生素 C 100，200和400 mg·kg -1处理后，抬高的 ST 段降低，OTM 值下降，心肌酶的活性降低，与纳米二氧化钛染毒组相比有显著性差异（P＜0．05）；病理组织学检查结果显示，心肌水肿减轻，炎性细胞减少，并增加心肌组织中的 SOD 和GSH-Px活性，与纳米二氧化钛处理组相比，差异有显著性（P＜0．05）。结论纳米二氧化钛可造成小鼠心肌损伤，维生素 C 可对抗纳米二氧化钛所致的心脏损伤，其机制可能与其增强心肌组织的抗氧化能力有关。
목적：관찰유생소 C 대납미이양화태염독소서심장손상적보호작용。방법곤명소서 ig 급여유생소 C 100，200화400 mg·kg -12 d，후3 d 간격4 h 후 ig 급여납미이양화태2 g·kg -1，말차염독후24 h진행지표검측。생리기록의측정심전도；생화법측정혈청중심기매활성급혈청화심기조직초양화물기화매（SOD）화곡광감태과양화매（GSH-Px）활성；혜성실험검사심기단세포 DNA 손상；HE 염색광경관찰심장조직병리개변。결과납미이양화태2 g·kg -1염독소서후，심전도검사발현 ST 단태고、T 파고첨，여정상대조조비차이유현저성（P ＜0．05）；염독조혈청중심기매활성균교정상대조조명현승고（P＜0．05），혈청화심기조직중 SOD 화 GSH-Px 활성강저（P＜0．05）。혜성실험결과표명，납미이양화태2 g·kg -1염독조혜성 Olive 미거（OTM）현저고우정상대조조（P＜0．05）。조직병이학결과현시，납미이양화태2 g·kg -1염독후소서심기조직출현세포수종、심기섬유문란、염성세포증다。급여유생소 C 100，200화400 mg·kg -1처리후，태고적 ST 단강저，OTM 치하강，심기매적활성강저，여납미이양화태염독조상비유현저성차이（P＜0．05）；병리조직학검사결과현시，심기수종감경，염성세포감소，병증가심기조직중적 SOD 화GSH-Px활성，여납미이양화태처리조상비，차이유현저성（P＜0．05）。결론납미이양화태가조성소서심기손상，유생소 C 가대항납미이양화태소치적심장손상，기궤제가능여기증강심기조직적항양화능력유관。
OBJECTIVE To observe the protection of vitamin C on the cardiac injury induced by 50 nm titanium dioxide inmice.METHODS Kunming mice were ad mistered by ig of vitamin C 100,200 and 400 mg·kg -1 for 2 d.And then the mice were ad mistered by ig of nano-TiO2 2 g·kg -1 and vitamin C (100.0,200.0 and 400.0 mg·kg -1 )for 3 d,the interval of treatment with nano-TiO2 and vitamin C was 4 h.The mice were scarified 24 h later after the last ad ministration.Electrocardiogra m (ECG)was determinated by physiological recorder.The myocardial enzy mes activities in serum and superoxide dismutase (SOD)and glutathione peroxidase(GSH-Px)activities in serum and myocardial tissue were determinated by bioche mical method.Cometassay was used to detect the DNA da mage of the heart. Heart tissue was used for histopathological exa mination by HE staining.RESULTS Co mpared with the control,ECG showed higher S-T and T-wave a mplitude of nano-TiO2 2 g·kg -1 (P<0.05).The myocar-dial enzy mes activities significantly increased and activities of SOD and GSH-Px significantly decreased in nano-TiO2 group,compared with the control group(P <0.05).Cometassay showed that olive tail mo ment (OTM)was significantly increased after nano-TiO2 2 g·kg -1 ,compared with the control group (P<0.05).The histopathology showed ede ma of myocardial cells,myofibril disorders and increasing infla mmatory cells.Vita min C 100,200 and 400 mg·kg -1 can decrease S-T in ECG,OTM,myocardial enzy mes activities,increase the SOD and GSH-Px activities in serum and myocardial tissue;reduce myocardial hypertrophy and infla mmatory cells.CONCLUSION nano-TiO2 can induce myocardial injury inmice and vitamin C can alleviate the da mage.The mechanism may be associated with the antioxidant ability of vitamin C inmyocardial tissue.