CAJ | 학술논문

背景：糖尿病性膀胱病是糖尿病患者最常见的慢性并发症之一，建立糖尿病膀胱病动物模型为相关研究提供实验动物平台。 　　目的：建立糖尿病膀胱病豚鼠模型并进行尿动力学评价。 　　方法：50只英国种短毛雌性豚鼠，实验组(n=42)以单次腹腔注射链脲佐菌素法诱导糖尿病豚鼠，对照组(n=8)注射相应剂量空白枸橼酸缓冲液，分别在9周和12周时行尿动力学检查，确定膀胱功能失代偿豚鼠即糖尿病性膀胱病组和代偿豚鼠即代偿组豚鼠尿动力学特点。 　　结果与结论：42只豚鼠有20只成功诱导出糖尿病。糖尿病豚鼠中，9周时9只糖尿病组豚鼠，6只膀胱功能代偿，3只失代偿；12周时，另外9只糖尿病组豚鼠，1只膀胱功能代偿，8只失代偿(89%)。糖尿病性膀胱病组豚鼠残余尿量增加(0.72±0.08) mL、最大逼尿肌压下降(0.63±0.05) kPa、膀胱容量增加(2.01±0.05) mL及膀胱顺应性增加(3.47±0.41) mL/kPa，与对照组及代偿组比较，差异均存在显著性意义(P <0.001)。豚鼠一般可在诱导糖尿病成功后12周发生糖尿病性膀胱病，表现出膀胱残余尿量增加等相应的尿动力学改变。
배경：당뇨병성방광병시당뇨병환자최상견적만성병발증지일，건립당뇨병방광병동물모형위상관연구제공실험동물평태。 　　목적：건립당뇨병방광병돈서모형병진행뇨동역학평개。 　　방법：50지영국충단모자성돈서，실험조(n=42)이단차복강주사련뇨좌균소법유도당뇨병돈서，대조조(n=8)주사상응제량공백구연산완충액，분별재9주화12주시행뇨동역학검사，학정방광공능실대상돈서즉당뇨병성방광병조화대상돈서즉대상조돈서뇨동역학특점。 　　결과여결론：42지돈서유20지성공유도출당뇨병。당뇨병돈서중，9주시9지당뇨병조돈서，6지방광공능대상，3지실대상；12주시，령외9지당뇨병조돈서，1지방광공능대상，8지실대상(89%)。당뇨병성방광병조돈서잔여뇨량증가(0.72±0.08) mL、최대핍뇨기압하강(0.63±0.05) kPa、방광용량증가(2.01±0.05) mL급방광순응성증가(3.47±0.41) mL/kPa，여대조조급대상조비교，차이균존재현저성의의(P <0.001)。돈서일반가재유도당뇨병성공후12주발생당뇨병성방광병，표현출방광잔여뇨량증가등상응적뇨동역학개변。
BACKGROUND:Diabetic cystopathy is one of the most common chronic diabetic complications. The establishment of animal models of diabetic cystopathy wil provide experimental animal platform for relevant research. OBJECTIVE:To establish a guinea pig model of diabetic cystopathy and to evaluate its urodynamic characteristics. METHODS:Fifty short-hair Britain female guinea pigs were randomly divided into two groups, 42 as the experiment group and the other 8 as the control group. The experiment group was intraperitoneal y injected with streptozotocin to induce diabetes. The control group received injection of blank citric acid buffered solution. Diabetic guinea pigs were detected by urinary dynamics test at 9 and 12 weeks. Diabetic guinea pigs were further assigned into diabetic cystopathy subgroup and compensated subgroup. The urodynamic parameters of three groups were compared. RESULTS AND CONCLUSION:Twenty of 42 guinea pigs were successful y induced diabetes by the injection of streptozotocin. At 9 weeks after the injection, bladder function compensation was present in six diabetic guinea pigs while bladder function was decompensated in another three diabetic guinea pigs. At 12 weeks, bladder function compensation was present in one diabetic guinea pig, while another eight guinea pigs were confirmed with diabetic cystopathy (88.89%). In the diabetic cystopathy subgroup, the residual urine volume was increased (0.72±0.08) mL, maximal detrusor pressure was decreased (0.63±0.05) kPa, maximum bladder capacity was increased (2.01±0.05) mL, and bladder compliance was increased (0.34±0.04) mL/kPa. There were significant differences compared with the compensated subgroup and the control group (P<0.001). Diabetic cystopathy occurs at 12 weeks after diabetic models are successful y established in guinea pigs, and urodynamic changes are mainly the increase of residual urine volume.