CAJ | 학술논문

为更好地研究水稻单倍体育种和转基因技术，加速水稻育种进程，以10份寒地水稻品种为试验材料，采用单因素试验方法，研究培养基的琼脂浓度、激素配比、马铃薯水提取液附加物添加及培养方式等因素对水稻花药培养愈伤组织诱导率的影响，优化寒地水稻品种花药组织培养条件。结果表明：10个品种花药组织培养，愈伤组织诱导率差异明显；在琼脂浓度为0．75％左右时，愈伤组织的诱导率达到最高；培养基中激素配比为KT（2 mg·L-1）＋IAA（1 mg·L-1）时诱导效果最佳；培养方式研究中，在双态培养方式下花药愈伤组织诱导率最好，其次为液态培养，再次为固态培养；培养基中添加马铃薯水提取液各处理间差异不显著。
위경호지연구수도단배체육충화전기인기술，가속수도육충진정，이10빈한지수도품충위시험재료，채용단인소시험방법，연구배양기적경지농도、격소배비、마령서수제취액부가물첨가급배양방식등인소대수도화약배양유상조직유도솔적영향，우화한지수도품충화약조직배양조건。결과표명：10개품충화약조직배양，유상조직유도솔차이명현；재경지농도위0．75％좌우시，유상조직적유도솔체도최고；배양기중격소배비위KT（2 mg·L-1）＋IAA（1 mg·L-1）시유도효과최가；배양방식연구중，재쌍태배양방식하화약유상조직유도솔최호，기차위액태배양，재차위고태배양；배양기중첨가마령서수제취액각처리간차이불현저。
In order to study rice haploid breeding and transgenic technology better and to accelerate the process of rice breeding ,taking 10 rice varieties in cold region as experimental materials ,single factor experiment was a-dopt to study the effect of agar concentration ,hormone conbination ,potatoes water extract ,culture method and other factors on callus induction rate of rice anther culture ,anther tissue culture conditions of rice varieties in cold region was optimized .The results showed that 10 varieties of anther was cultured and the difference of an-ther callus induction rate was obvious .Agar concentration was about 0 .75% ,the callus induction rate reached the highest .When the hormone combination was KT (2 mg·L-1 )+ IAA (1 mg·L-1 ) ,induction effect was the best .The callus induction rate of double layer’s medium culture was the best followed by liquid culture ,again for solid culture .Potatoes water extract addition showed there was no significant difference in different treat-ments .