CAJ | 학술논문

基于脲酶（ UASE）催化尿素水解的反应中Cu2＋对于酶的抑制作用原理，通过测定产物吸光度的变化率得出了其酶促反应速度，对应于Cu2＋浓度而制得标准曲线，检出限为0．05μmol/L．讨论了pH值和温度对测定的影响，结果表明：该法快速灵敏．
기우뇨매（ UASE）최화뇨소수해적반응중Cu2＋대우매적억제작용원리，통과측정산물흡광도적변화솔득출료기매촉반응속도，대응우Cu2＋농도이제득표준곡선，검출한위0．05μmol/L．토론료pH치화온도대측정적영향，결과표명：해법쾌속령민．
A spectroscopic method was developed to determine copper (Ⅱ) content based on the inhibition of copper (Ⅱ) for the hydrolysis of urea catalyzed by urease ( UASE) .The rate of the absorbance change of the product was measured for the calculation of the rate of the enzymatic reaction and a standard curve of copper (Ⅱ) was prepared .The detection limit could reach 0.05μmol/L, and the effects of the pH value and temperature were also discussed .The results indicated that the method was rapid and sensitive .