CAJ | 학술논문

为探究环境雌激素对鱼类早期发育的毒性作用，采用生化和静态急性毒性试验方法，研究了瓯江彩鲤早期发育过程中碱性磷酸酶和酸性磷酸酶活性的变化及间甲酚对瓯江彩鲤早期发育中磷酸酶活性的影响。试验结果表明，瓯江彩鲤受精卵中磷酸酶活性均低于成熟卵，卵裂期酶活性最低；在水温（20±2）℃条件下，经7、13、19 mg/L的间甲酚暴露，各质量浓度组磷酸酶活性表现为良好的剂量-效应和发育时期-效应关系。总体来说，瓯江彩鲤从原肠胚期开始，磷酸酶活性随着发育时间的延长和暴露质量浓度的增加而升高。
위탐구배경자격소대어류조기발육적독성작용，채용생화화정태급성독성시험방법，연구료구강채리조기발육과정중감성린산매화산성린산매활성적변화급간갑분대구강채리조기발육중린산매활성적영향。시험결과표명，구강채리수정란중린산매활성균저우성숙란，란렬기매활성최저；재수온（20±2）℃조건하，경7、13、19 mg/L적간갑분폭로，각질량농도조린산매활성표현위량호적제량-효응화발육시기-효응관계。총체래설，구강채리종원장배기개시，린산매활성수착발육시간적연장화폭로질량농도적증가이승고。
Activities of acid phosphatase ( ACP) and alkaline phosphatase ( ALP) were determined and the effect of m-cresol on the activities of ACP and ALP was studied during early development of common carp Cyprinus carpio var .color by biochemical and static toxicological methods to evaluate the toxic effect of environmental estrogens on early development of fish . The results showed that the activities of phosphatase were lower in the fertilized eggs than those in the mature eggs ,with the minimal value in cleavage stage of the fertilized eggs exposed to the m-cresol at concentration of 7 mg/L ,13 mg/L and 19 mg/L at water temperature of (20 ± 2) ℃ .There was a close dose-effect and development stage-effect relationship for the activities of ALP and ACP in each concentration group .In general ,the activities of ALP and ACP were found to be increased with the development and the increase in m-cresol concentration during different early development stages of the common carp .