CAJ | 학술논문

为提高海蜇的综合利用率，利用木瓜蛋白酶酶解技术制备海蜇生殖腺酶解物。分析酶解物的氨基酸组成，并通过体外化学模拟体系评价酶解物与F e2＋螯合前后的抗氧化活性。试验结果表明，海蜇生殖腺酶解物含有17种氨基酸，具有一定的Fe2＋螯合能力和DPPH ·清除能力，其螯合率和清除率达到50％时的质量浓度分别为1．73 mg/mL 和22．20 mg/mL。同时，具有一定的还原能力，其在700 nm处吸光值达到0．5时的质量浓度为17．61 mg/mL。海蜇生殖腺酶解物与Fe2＋螯合后，抗氧化能力显著增强，DPPH ·清除率达到50％和在700 nm处吸光值达到0．5时的质量浓度分别为0．15 mg/mL和0．86 mg/mL。
위제고해철적종합이용솔，이용목과단백매매해기술제비해철생식선매해물。분석매해물적안기산조성，병통과체외화학모의체계평개매해물여F e2＋오합전후적항양화활성。시험결과표명，해철생식선매해물함유17충안기산，구유일정적Fe2＋오합능력화DPPH ·청제능력，기오합솔화청제솔체도50％시적질량농도분별위1．73 mg/mL 화22．20 mg/mL。동시，구유일정적환원능력，기재700 nm처흡광치체도0．5시적질량농도위17．61 mg/mL。해철생식선매해물여Fe2＋오합후，항양화능력현저증강，DPPH ·청제솔체도50％화재700 nm처흡광치체도0．5시적질량농도분별위0．15 mg/mL화0．86 mg/mL。
The enzymatic hydrolysates of gonad in jellyfish Rhopilema esculentum were prepared by papain ,and the amino acid composition and the antioxidant activities of the hydrolysates were analyzed by Fe2+-chelating assay ,1 ,1-diphenyl-2-picrylhydrazyl (DPPH · ) assay and reducing power assay to make full use of the jellyfish .The results showed that the hydrolysates from jellyfish gonad were composed of 17 amino acids ,and possessed Fe2+ -chelatin ability ,DPPH radical scavenging capacity with IC50 values of 1 . 73 and 22 .20 mg/mL , respectively , and reducing power with AC0 .5 value of 17 .61 mg/mL .The antioxidant activities of enzymatic hydrolysates ,chelated with Fe2+ ,were obviously improved ,with the IC50 and AC0 .5 values of 0 .15 mg/mL in DPPH radical scavenging capacity and 0 .86 mg/mL in reducing power .The findings provide theoretical support for the further exploitation of jellyfish gonad .