安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
3期
363-365,366
,共4页
胃癌%MIF%MMP-9%免疫组织化学
胃癌%MIF%MMP-9%免疫組織化學
위암%MIF%MMP-9%면역조직화학
gastric cancer%MIF%MMP-9%immunohistochemistry
目的探讨人胃癌组织及其癌旁正常组织中巨噬细胞移动抑制因子( MIF )和基质金属蛋白酶9( MMP-9)的表达及临床意义。方法选取手术切除并经病理证实的胃癌标本80例作为实验组,另取80例相应癌旁组织作为对照组。应用免疫组化SP法检测癌组织及其癌旁正常组织标本中MIF、MMP-9的表达。结果[1]胃癌组织中MIF、MMP-9有不同程度的表达, MIF、MMP-9二者的阳性率分别为68.8%和67.5%,均高于癌旁正常组织中的32.5%和37.5%,差异有统计学意义( P<0.05);[2]MIF的表达与肿瘤浸润深度、分化程度、淋巴结转移及 TNM 分期呈正相关(P<0.05),与其他临床病理参数无关; MMP-9与肿瘤浸润深度、淋巴结转移及TNM分期呈正相关( P<0.05),与其他临床病理参数无关;[3] MIF与MMP-9的表达存在关联(χ2=9.154, P<0.01,r=0.338)。结论胃癌组织中MIF与MMP-9均呈阳性表达,且二者的表达呈正相关;二者在胃癌组织中不同程度的表达与胃癌的发生、发展密切相关,并可能指导胃癌的诊断、治疗及预后。
目的探討人胃癌組織及其癌徬正常組織中巨噬細胞移動抑製因子( MIF )和基質金屬蛋白酶9( MMP-9)的錶達及臨床意義。方法選取手術切除併經病理證實的胃癌標本80例作為實驗組,另取80例相應癌徬組織作為對照組。應用免疫組化SP法檢測癌組織及其癌徬正常組織標本中MIF、MMP-9的錶達。結果[1]胃癌組織中MIF、MMP-9有不同程度的錶達, MIF、MMP-9二者的暘性率分彆為68.8%和67.5%,均高于癌徬正常組織中的32.5%和37.5%,差異有統計學意義( P<0.05);[2]MIF的錶達與腫瘤浸潤深度、分化程度、淋巴結轉移及 TNM 分期呈正相關(P<0.05),與其他臨床病理參數無關; MMP-9與腫瘤浸潤深度、淋巴結轉移及TNM分期呈正相關( P<0.05),與其他臨床病理參數無關;[3] MIF與MMP-9的錶達存在關聯(χ2=9.154, P<0.01,r=0.338)。結論胃癌組織中MIF與MMP-9均呈暘性錶達,且二者的錶達呈正相關;二者在胃癌組織中不同程度的錶達與胃癌的髮生、髮展密切相關,併可能指導胃癌的診斷、治療及預後。
목적탐토인위암조직급기암방정상조직중거서세포이동억제인자( MIF )화기질금속단백매9( MMP-9)적표체급림상의의。방법선취수술절제병경병리증실적위암표본80례작위실험조,령취80례상응암방조직작위대조조。응용면역조화SP법검측암조직급기암방정상조직표본중MIF、MMP-9적표체。결과[1]위암조직중MIF、MMP-9유불동정도적표체, MIF、MMP-9이자적양성솔분별위68.8%화67.5%,균고우암방정상조직중적32.5%화37.5%,차이유통계학의의( P<0.05);[2]MIF적표체여종류침윤심도、분화정도、림파결전이급 TNM 분기정정상관(P<0.05),여기타림상병리삼수무관; MMP-9여종류침윤심도、림파결전이급TNM분기정정상관( P<0.05),여기타림상병리삼수무관;[3] MIF여MMP-9적표체존재관련(χ2=9.154, P<0.01,r=0.338)。결론위암조직중MIF여MMP-9균정양성표체,차이자적표체정정상관;이자재위암조직중불동정도적표체여위암적발생、발전밀절상관,병가능지도위암적진단、치료급예후。
Objective To investigate the expression and clinical significance of migration inhibitory factor ( MIF) and matrix metalloproteinase-9(MMP-9) in human gastric cancer tissue and corresponding adjacent normal tissue. Methods Selected surgery and pathologically confirmed 80 cases of gastric cancer as the experimental group, took another 80 cases of corresponding adjacent tissues as a control group. The expressions of MIF and MMP-9 were de-tected in 80 cases of gastric cancer patients cancer tissue and corresponding adjacent normal tissue by immunohisto-chemical SP method. Results ①The expression of MIF and MMP-9 of gastric cancer in varying degrees:The pos-itive rates of MIF and MMP-9 were 68. 8% and 67. 5%,which were higher than that in adjacent normal tissues, 32. 5% and 37.5% (P <0.05);② The expression of MIF was significantly associated with the clinical stage, depth of invasion,degree of differentiation,lymph node metastasis and TNM staging (P<0.05), nothing to do with other clinicopathological parameters;the expression of MMP-9 was significantly associated with the depth of inva-sion,degree of differentiation, lymph node metastasis and TNM staging (P<0.05), nothing to do with other clini-copathological parameters;③ There was positive correlation between the expression of MIF and MMP-9 in gastric cancer tissue (χ2 =9. 154, P<0. 01,r=0.338). Conclusion The expressions of MIF and MMP-9 show a posi-tive expression in gastric carcinoma, there maybe have a positive correlation between MIF and MMP-9. Their ex-pressions of varying degrees are closely related with the occurrence and development of gastric cancer, guidance gastric diagnosis, treatment and prognosis.