CAJ | 학술논문

目的研究NEDD4-1基因在正常皮肤与增生性瘢痕和瘢痕疙瘩中的表达情况,探讨其在病理性瘢痕发病机制中的作用。方法收集临床标本30例,其中增生性瘢痕10例(A组)、瘢痕疙瘩10例(B组)、正常皮肤10例(C组),采用Western blot 法和普通 RT-PCR 方法检测 A、B、C 3组中NEDD4-1蛋白和mRNA表达情况,并进行统计学分析。结果在A、B组中,NEDD4-1 mRNA和蛋白均可表达,但表达差异无统计学意义(P>0.05),两组分别与C组比较,差异无统计学意义( P>0.05)。结论 NEDD4-1基因在病理性瘢痕中均可表达,但NEDD4-1的表达与病理性瘢痕的发生机制之间的关系仍需进一步研究。
목적연구NEDD4-1기인재정상피부여증생성반흔화반흔흘탑중적표체정황,탐토기재병이성반흔발병궤제중적작용。방법수집림상표본30례,기중증생성반흔10례(A조)、반흔흘탑10례(B조)、정상피부10례(C조),채용Western blot 법화보통 RT-PCR 방법검측 A、B、C 3조중NEDD4-1단백화mRNA표체정황,병진행통계학분석。결과재A、B조중,NEDD4-1 mRNA화단백균가표체,단표체차이무통계학의의(P>0.05),량조분별여C조비교,차이무통계학의의( P>0.05)。결론 NEDD4-1기인재병이성반흔중균가표체,단NEDD4-1적표체여병이성반흔적발생궤제지간적관계잉수진일보연구。
Objective To examine the gene expression of NEDD4-1 in pathological scar and explore its effect on formation of pathological scar. Methods Tissue specimens were collected from 10 cases of hypertrophic scar ( group A) , 10 cases of keloid ( group B) and 10 cases of normal skin ( group C) respectively. Western blot and RT-PCR techniques were performed to detect the protein and gene expression of NEDD4-1 . Results NEDD4-1 mRNA and protein in both group A and B could be expressed, while the difference between the two groups was not significant (P>0.05). Moreover, compared with the group C, the difference in gene expression of NEDD4-1 did not reach statistical significance in both group A and group B (P>0.05). Conclusion In our experimental study, NEDD4-1 gene is present in pathological scar, but further studies in the detailed mechanism are warranted.