CAJ | 학술논문

以微球菌核酸酶法分离的啤酒酵母(Saccharomyces cerevisiae)染色体单核小体DNA为模板进行PCR,根据不同靶序列PCR产物拷贝数的不同定位核小体位置。结果表明,啤酒酵母Ⅲ号染色体ARS305附近核小体位置大约在39107位到39269位、39318位到39470位及39540位到39694位之间,理论计算结果与试验结果基本一致。
이미구균핵산매법분리적비주효모(Saccharomyces cerevisiae)염색체단핵소체DNA위모판진행PCR,근거불동파서렬PCR산물고패수적불동정위핵소체위치。결과표명,비주효모Ⅲ호염색체ARS305부근핵소체위치대약재39107위도39269위、39318위도39470위급39540위도39694위지간,이론계산결과여시험결과기본일치。
Using the variation pattern of copy number of PCR product as a function of PCR target sequence, position to the nucleosome locations was determined when isolated mono-nucleosome DNA of Saccharomyces cerevisiae by micrococcal nuclease was used as the PCR template. The results showed that there were three nucleosomes around ARS305 of chromosome Ⅲ located at 39107- 39269 bp, 39318-39470 bp, and 39540- 39694 bp. Computational calculation was in agreement with the experimental data.