CAJ | 학술논문

目的：探讨胃癌患者术中腹腔冲洗液的 MMP-2mRNA、MMP-14mRNA 在胃癌腹腔转移中的临床意义。方法应用 RT-PCR 法检查98例原发性胃癌腹腔冲洗液中 MMP-2mRNA、MMP-14mRNA 的表达，30例良性病变病人腹腔冲洗液作为阴性对照。结果胃癌患者腹腔冲洗液中MMP-2mRNA、MMP-14mRNA阳性表达率分别为42/98（42．9％）、40/98（40．8％），分别高于对照组的2/30（6．67％）、3．33％（1/30）（P ＜0．01）。MMP-2mRNA、MMP-14mRNA 的表达阳性率随着肿瘤浸润深度的加深而显著增加，与腹腔内是否转移及胃癌的大体类型有关（P ＜0．01），两者呈正相关。结论采用 RT-PCR技术检测胃癌患者腹腔冲洗液中的 MMP-2mRNA 及 MMP-14mRNA 表达，可以用于预测胃癌患者腹腔转移。同时也说明MMP-2及 MMP-14参与了胃癌的腹腔转移的过程。
목적：탐토위암환자술중복강충세액적 MMP-2mRNA、MMP-14mRNA 재위암복강전이중적림상의의。방법응용 RT-PCR 법검사98례원발성위암복강충세액중 MMP-2mRNA、MMP-14mRNA 적표체，30례량성병변병인복강충세액작위음성대조。결과위암환자복강충세액중MMP-2mRNA、MMP-14mRNA양성표체솔분별위42/98（42．9％）、40/98（40．8％），분별고우대조조적2/30（6．67％）、3．33％（1/30）（P ＜0．01）。MMP-2mRNA、MMP-14mRNA 적표체양성솔수착종류침윤심도적가심이현저증가，여복강내시부전이급위암적대체류형유관（P ＜0．01），량자정정상관。결론채용 RT-PCR기술검측위암환자복강충세액중적 MMP-2mRNA 급 MMP-14mRNA 표체，가이용우예측위암환자복강전이。동시야설명MMP-2급 MMP-14삼여료위암적복강전이적과정。
Objective To investigate the clinical significance of detecting MMP-2mRNA and MMP-14mRNA with peritoneal lavage fluid in patients with gastric cancer.Methods The ex-pression of 98 patients of MMP-2mRNA and MMP-14 mRNA in peritoneal lavage fluid were de-teced by RT-PCR in patients with gastric cancer.30 patients with benign peritoneal lavage fluid were in the negative control group.Results The positive expression rates of MMP-2mRNA and MMP-14mRNA in the peritoneal lavage fluid in patients with gastric cancer were 42 /98(42.9%) and 40 /98(40.8%).but they were 2 /30(6.67%)、3.33% (1 /30)respectively in normal control (P <0.01).The positive expression rate of MMP-2mRNA and MMP-14mRNA were related to the depth of invasion,peritoneal metastasis,macroscopic type (P <0.01).Conclusion RT-PCR de-tection on the expression of MMP-2mRNA and MMP-14mRNA in peritoneal lavage fluid in patients with gastric cancer can be used to predict the peritoneal metastases.MMP-2 and MMP-14 may be involved in the peritoneal metastasis process.