CAJ | 학술논문

目的：探讨依达拉奉对犬自体肾移植外周血中炎性因子的影响。方法将12只体质量匹配的健康杂种犬随机分为依达拉奉组（ED 组）和生理盐水组（PS 组），每组6只。ED 组术中静脉内给予依达拉奉10 mg /kg，术后第1、2、3天各予依达拉奉10 mg /kg；PS 组同法使用相同体积的生理盐水。分别在麻醉前（T0）、术中（T1）、术后1 d（T2）与术后3 d（T3）经静脉采血检测 TNF-α、IL-6、IL-8、IL-10的水平。结果2组外周血 T0期比较各因子浓度无显著差别；T1、T2、T3各期 TNF-α、IL-6、IL-8、IL-10浓度较 T0期显著升高（P ＜0．05），T1期剧升，T2期达到高峰，T3期略回落但仍高于 T0期；ED 组 TNF-α、IL-6、IL-8升高的幅度在 T1、T2、T3期小于 PS 组（P ＜0．05）；2组 IL-10比较无显著差异（P ＞0．05）。结论肾移植炎性因子显著升高，可能与肾移植缺血再灌注损伤有关；依达拉奉能减少炎性因子的释放，亦是其肾保护作用的机制。
목적：탐토의체랍봉대견자체신이식외주혈중염성인자적영향。방법장12지체질량필배적건강잡충견수궤분위의체랍봉조（ED 조）화생리염수조（PS 조），매조6지。ED 조술중정맥내급여의체랍봉10 mg /kg，술후제1、2、3천각여의체랍봉10 mg /kg；PS 조동법사용상동체적적생리염수。분별재마취전（T0）、술중（T1）、술후1 d（T2）여술후3 d（T3）경정맥채혈검측 TNF-α、IL-6、IL-8、IL-10적수평。결과2조외주혈 T0기비교각인자농도무현저차별；T1、T2、T3각기 TNF-α、IL-6、IL-8、IL-10농도교 T0기현저승고（P ＜0．05），T1기극승，T2기체도고봉，T3기략회락단잉고우 T0기；ED 조 TNF-α、IL-6、IL-8승고적폭도재 T1、T2、T3기소우 PS 조（P ＜0．05）；2조 IL-10비교무현저차이（P ＞0．05）。결론신이식염성인자현저승고，가능여신이식결혈재관주손상유관；의체랍봉능감소염성인자적석방，역시기신보호작용적궤제。
Obj ective To explore the influence of edaravone on inflammatory factor of pe-ripheral blood in canine with renal auto-transplantation.Methods 12 weight matched healthy mon-grel dogs were randomly divided into edaravone group (ED group )and normal saline group (PS group),6 dogs in each group.ED group was treated with edaravone by 10 mg/kg intravenous in in-traoperative,postoperative 1,2,3 days,and each for edaravone 10 mg/kg.PS group was treated with the method of using the same volume of saline.The levels of TNF-α,IL-6,IL-8,IL-10 were detected by sampling venous blood before anesthesia (T0 )and after surgery (T1 ),1 days (T2 )and 3 days (T3 )after operation respectively.Results Compared with levels of inflammatory factors of peripheral blood in T0 period,there were no significant differences of concentration in each factor. With increasing of concentration of IL-6,IL-8,TNF-α,IL-10 at T1,T2,T3(P<0.05),the lev-els raised at T1 ,reached a peak at T2 ,slightly down at T3 but still higher than T0 .Levels of TNF-α,IL-6 ,IL-8 in ED group increased in T1 ,T2 ,T3 were significantly less than the PS group (P<0.05).There was no significant difference of IL-10 between two groups (P>0.05).Conclusion Inflammatory factors significantly increase in dogs with renal allograft,and it may be associated with ischemia reperfusion inj ury in renal transplantation.Edaravone can reduce the release of in-flammatory factors,which is also its mechanism of renal protection effect.