CAJ | 학술논문

目的：通过对肝细胞肝癌组织中 VEGF 和 DLL4表达水平的测定，分析其与临床病理分期的关系及意义。方法2010年1月-2013年6月本院外科手术切除并经病理证实的肝细胞肝癌组织石蜡标本75例，采用免疫组化 SP 法测定75例肝细胞肝癌及30例癌旁肝组织中 VEGF 和 DLL4的表达水平。结果VEGF 和 DLL4在肝细胞肝癌组织中表达的阳性率分别为64．0％、81．3％，明显高于癌旁组织（P ＜0．05）。VEGF 及 DLL4在肝细胞肝癌中的表达与肿瘤数目、有无静脉浸润密切相关（P ＜0．05）；两者在肝细胞肝癌组织中的表达与患者性别、年龄、肝硬化程度、肿瘤直径和分化程度无关（P ＞0．05）。VEGF阳性表达患者中的 DLL4表达率明显高于 VEGF 阴性表达患者，VEGF 和 DLL4表达水平呈显著正相关（r ＝0．217，P ＜0．05）。结论VEGF 和 DLL4在肝细胞肝癌和正常肝细胞中的表达差异有统计学意义，两者在肝细胞肝癌中表达上调具有一致性，共同促使肝癌新生血管生发，参与肝细胞肝癌的生长和转移，影响患者的预后。
목적：통과대간세포간암조직중 VEGF 화 DLL4표체수평적측정，분석기여림상병리분기적관계급의의。방법2010년1월-2013년6월본원외과수술절제병경병리증실적간세포간암조직석사표본75례，채용면역조화 SP 법측정75례간세포간암급30례암방간조직중 VEGF 화 DLL4적표체수평。결과VEGF 화 DLL4재간세포간암조직중표체적양성솔분별위64．0％、81．3％，명현고우암방조직（P ＜0．05）。VEGF 급 DLL4재간세포간암중적표체여종류수목、유무정맥침윤밀절상관（P ＜0．05）；량자재간세포간암조직중적표체여환자성별、년령、간경화정도、종류직경화분화정도무관（P ＞0．05）。VEGF양성표체환자중적 DLL4표체솔명현고우 VEGF 음성표체환자，VEGF 화 DLL4표체수평정현저정상관（r ＝0．217，P ＜0．05）。결론VEGF 화 DLL4재간세포간암화정상간세포중적표체차이유통계학의의，량자재간세포간암중표체상조구유일치성，공동촉사간암신생혈관생발，삼여간세포간암적생장화전이，영향환자적예후。
Objective To explore the expression of VEGF and DLL4 in hepato cellular carci-noma tissues.Methods Expressions of VEGF and DLL4 in 75 cases of hepatocellular carcinoma tis-sues and 30 cases of normal hepatocellular mucosa tissues were detected by immunohistochemical SP method.Results The masculine expression rates of VEGF and DLL4 in hepatocellular carcinoma tis-sues were 64.0% and 81.3% respectively.The positive expression rates of VEGF and DLL4 in hepa-tocellular carcinoma were higher than those in normal hepatocellular mucosa.Expressions of VEGF and DLL4 in hepatocellular carcinoma tissues were associated with vascular invasion and tumor numbers(P<0.05),but not with gender,age,liver cirrhosis,the diameter of tumor and the histological differen-tiation(P >0.05 ).The VEGF expression rate in DLL4 positive expression cases was significantly higher than DLL4 negative cases.VEGF had a positive correlation with DLL4 (r =0.217,P <0.05). Conclusion The expressions of VEGF and DLL4 in hepatocellular carcinoma and normal tissues are different.The increased expression of VEFG in hepatocellular carcinoma is closely related to DLL4 and they probably play an important role in tumor angiogenesis.