CAJ | 학술논문

目的：探讨灯盏花素（Br）对脑缺血再灌注大鼠海马核因子（NF）-κB表达的影响。方法采用Zea-Longa法建立脑缺血再灌注动物模型。96只SD大鼠根据不同的处理因素分为：假手术组（Sham组，32只）、灯盏花素治疗组（Br组，32只）、缺血再灌注组（I/R组，32只）。Br组和 I/R组依据缺血后再灌注的不同时间点再细分为3、12、24、48 h 4个小组。应用免疫组织化学方法和蛋白印迹方法检测各组大鼠海马N F-κB蛋白的表达。结果与假手术组相比，在缺血再灌注3 h时，大鼠海马N F-κB的阳性表达已明显升高，在12 h时表达最高，随后表达开始下降（P＜0.05）；与相同时间点的缺血再灌注组大鼠比较，3 h时，Br组的NF-κB蛋白阳性表达未见明显降低（P＞0.05），其余各时间点则明显降低（P＜0.05）；蛋白印迹方法也得到了相同的结论。结论灯盏花素很可能通过减少NF-κB蛋白的表达改善脑缺血再灌注损伤。
목적：탐토등잔화소（Br）대뇌결혈재관주대서해마핵인자（NF）-κB표체적영향。방법채용Zea-Longa법건립뇌결혈재관주동물모형。96지SD대서근거불동적처리인소분위：가수술조（Sham조，32지）、등잔화소치료조（Br조，32지）、결혈재관주조（I/R조，32지）。Br조화 I/R조의거결혈후재관주적불동시간점재세분위3、12、24、48 h 4개소조。응용면역조직화학방법화단백인적방법검측각조대서해마N F-κB단백적표체。결과여가수술조상비，재결혈재관주3 h시，대서해마N F-κB적양성표체이명현승고，재12 h시표체최고，수후표체개시하강（P＜0.05）；여상동시간점적결혈재관주조대서비교，3 h시，Br조적NF-κB단백양성표체미견명현강저（P＞0.05），기여각시간점칙명현강저（P＜0.05）；단백인적방법야득도료상동적결론。결론등잔화소흔가능통과감소NF-κB단백적표체개선뇌결혈재관주손상。
Objective To study the expression of NF-κB in hippocampus of cerebral ischemia-reperfusion rat after breviscapine was injected .Methods The ischemia-reperfusion model was induced with Zea-Longa′s way . Ninty-six SD rats according to the different treatment were randomly divided into the sham group ,ischemia-reper-fusion group(I/R) and breviscapine group(Br) at different time points (3 h ,12 h ,24 h ,48 h) after reperfusion on average .The expression of NF-κB in hippocampus of all groups was examined by immunochemistry and west-ern blot methods .Results Compared with the sham group ,the positive expression of NF-κB in hippocampus of the rats in I/R group was increased significantly at the point of 3 h after reperfusion (P<0.05) ,to the peak at 12 h;compared with the rats in I/R group at the same time point ,the positive expression of NF-κB didn′t evidently reduce at the point of 3 h after breviscapine was injected (P>0.05) ,and decreased significantly in 12 h ,24 h and 48 h groups (P< 0.05);and the same result was obtained by western blot method .Conclusion Breviscapine could down-regulate the expression of NF-κB in hippocampus of cerebral ischemia-reperfusion rat .