中成药
中成藥
중성약
CHINESE TRADITIONAL PATENT MEDICINE
2013年
6期
1243-1247
,共5页
赵世元%农智新%叶海洪%钟振国%张明艳%李彩萍
趙世元%農智新%葉海洪%鐘振國%張明豔%李綵萍
조세원%농지신%협해홍%종진국%장명염%리채평
夜香树%甾体皂苷%抗肿瘤活性
夜香樹%甾體皂苷%抗腫瘤活性
야향수%치체조감%항종류활성
目的 分离并鉴定夜香树叶的甾体皂苷并测试其抗肿瘤活性.方法 运用反复硅胶柱层析的方法对夜香树叶正丁醇部位进行分离纯化,以LC-MS光谱数据确定其结构；采用MTT法评估分离物对3种肿瘤细胞株的毒性.结果 从夜香树叶的正丁醇部位中分得3个化合物,化合物1为spirost-5-ene-23-diol[2α,3β,25R 3-O-[β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl{(3→1)-β-D-xylopyranosyl}(1→4)-β-D-galactopyranoside；化合物2为spirost-5-ene-3-diol[3β,25R] 3-O-[{ α-L-rhamnopyranosyl-(1→4)α-L-rhamnopyranosyl-(1-→2)}-α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside;化合物3为spirost-5-ene-23-diol[2α,3β,25R] 3-O-[β-D-glu\opyranosyl-(1→3)-β-D-xylopyranosyl}(1→4)-β-D-galactopyranoside.3种皂苷均可以抑制肿瘤细胞BEL7404、HeLa和A549细胞的生长,并呈现一定剂量依赖关系和时间依赖关系.结论 夜香树甾体皂苷体外对3种肿瘤细胞的抑制作用强度可能取决于鼠李糖苷和吡喃葡萄糖苷的数量与甾体皂苷3号碳上的连接方式.
目的 分離併鑒定夜香樹葉的甾體皂苷併測試其抗腫瘤活性.方法 運用反複硅膠柱層析的方法對夜香樹葉正丁醇部位進行分離純化,以LC-MS光譜數據確定其結構；採用MTT法評估分離物對3種腫瘤細胞株的毒性.結果 從夜香樹葉的正丁醇部位中分得3箇化閤物,化閤物1為spirost-5-ene-23-diol[2α,3β,25R 3-O-[β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl{(3→1)-β-D-xylopyranosyl}(1→4)-β-D-galactopyranoside；化閤物2為spirost-5-ene-3-diol[3β,25R] 3-O-[{ α-L-rhamnopyranosyl-(1→4)α-L-rhamnopyranosyl-(1-→2)}-α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside;化閤物3為spirost-5-ene-23-diol[2α,3β,25R] 3-O-[β-D-glu\opyranosyl-(1→3)-β-D-xylopyranosyl}(1→4)-β-D-galactopyranoside.3種皂苷均可以抑製腫瘤細胞BEL7404、HeLa和A549細胞的生長,併呈現一定劑量依賴關繫和時間依賴關繫.結論 夜香樹甾體皂苷體外對3種腫瘤細胞的抑製作用彊度可能取決于鼠李糖苷和吡喃葡萄糖苷的數量與甾體皂苷3號碳上的連接方式.
목적 분리병감정야향수협적치체조감병측시기항종류활성.방법 운용반복규효주층석적방법대야향수협정정순부위진행분리순화,이LC-MS광보수거학정기결구；채용MTT법평고분리물대3충종류세포주적독성.결과 종야향수협적정정순부위중분득3개화합물,화합물1위spirost-5-ene-23-diol[2α,3β,25R 3-O-[β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl{(3→1)-β-D-xylopyranosyl}(1→4)-β-D-galactopyranoside；화합물2위spirost-5-ene-3-diol[3β,25R] 3-O-[{ α-L-rhamnopyranosyl-(1→4)α-L-rhamnopyranosyl-(1-→2)}-α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside;화합물3위spirost-5-ene-23-diol[2α,3β,25R] 3-O-[β-D-glu\opyranosyl-(1→3)-β-D-xylopyranosyl}(1→4)-β-D-galactopyranoside.3충조감균가이억제종류세포BEL7404、HeLa화A549세포적생장,병정현일정제량의뢰관계화시간의뢰관계.결론 야향수치체조감체외대3충종류세포적억제작용강도가능취결우서리당감화필남포도당감적수량여치체조감3호탄상적련접방식.
