CAJ | 학술논문

以白色和蓝色品种的非洲紫罗兰舌状花瓣为材料，测定其细胞液pH值，并利用RT-PCR技术，以NCBI上登记的马铃薯、矮牵牛、龙胆草、金鱼草和瓜叶菊的F3‘5＇H基因片段保守区域为模板，克隆乃芍，爿基因片段，为花色显色机理研究提供理论依据。实验结果表明：蓝色品种非洲紫罗兰细胞液的pH值大于白色品种非洲紫罗兰花瓣细胞液的pH值，随着花期的延长，两个品种非洲紫罗兰细胞液的pH值变化不断增大，且白色品种非洲紫罗兰花瓣细胞液的pH值变化大于蓝色品种非洲紫罗兰花瓣细胞液的pH值；其范围均在3．0-7．0之间，呈弱酸；在两种花色品种非洲紫罗兰中，都得到了F3‘5＇H基因片段的cDNA序列长度为392bp，与马铃薯、矮牵牛、龙胆草、金鱼草和瓜叶菊的F3‘5＇H基因保守区域片段的同源性分别为63．O％、55．4％、52．8％、49．0％和47．7％。
이백색화람색품충적비주자라란설상화판위재료，측정기세포액pH치，병이용RT-PCR기술，이NCBI상등기적마령서、왜견우、룡담초、금어초화과협국적F3‘5＇H기인편단보수구역위모판，극륭내작，장기인편단，위화색현색궤리연구제공이론의거。실험결과표명：람색품충비주자라란세포액적pH치대우백색품충비주자라란화판세포액적pH치，수착화기적연장，량개품충비주자라란세포액적pH치변화불단증대，차백색품충비주자라란화판세포액적pH치변화대우람색품충비주자라란화판세포액적pH치；기범위균재3．0-7．0지간，정약산；재량충화색품충비주자라란중，도득도료F3‘5＇H기인편단적cDNA서렬장도위392bp，여마령서、왜견우、룡담초、금어초화과협국적F3‘5＇H기인보수구역편단적동원성분별위63．O％、55．4％、52．8％、49．0％화47．7％。
The experiment used white and blue Saintpqulia ionantha cultivars＇ linguoid petal as test materials to determine their pH values of cellular sap and clone F3＇5＇H gene fragment which was compared with the F3＇5＇H genes ofSolanum tuberosum, Petunia hybrida, gentiana scabra bge, Snapdragon, Pericallis cruentia that were registered in NCBI by using the RT-PCR technique for the study of coloration mechanism. The results indicate that the pH value in blue Sain~qulia ionantha eultivar＇ cellular sap was obviously higher than that in white Sain~oqulia ionantha eultivar＇ cellular sap, and the pH values of two Saintpqulia ionantha eultivars petal cell showed increasing trend, the changes of pH value were greater in white Sainq~qulia ionantha cultivar＇ cellular sap than those in blue SainOgqulia ionantha cultivar＇ cellular sap. And the range of their pH values was between 3.0 and 7.0, which was faintly acid. In two Saintpqulia ionantha cultivars, the eDNA sequence of F3＇5＇Hgene fragment were both 392 bp. Compared with the F3＇5＇H genes, conservative area of Solanum tuberosum, Petunia hybrida, gentiana scabra bge, Snapdragon, Pericallis cruentia, the homology of nueleotide sequence separately reached 63.0%, 55.4%, 52.8%, 49.0% and 47.7%.