中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2013年
24期
1540-1543
,共4页
抑瘤素M%达卡巴嗪%黑色素瘤
抑瘤素M%達卡巴嗪%黑色素瘤
억류소M%체잡파진%흑색소류
oncostatin M%DTIC%melanoma
#目的：通过体内外实验探讨抑瘤素M（OSM）联合达卡巴嗪（DTIC）对小鼠黑色素瘤细胞B16的抑制作用。方法：在体外分别采用MTT法和FCM法检测达卡巴嗪单药以及联合OSM对黑色素瘤B16细胞增殖和凋亡的影响；Hochest染色法检测达卡巴嗪单药以及联合OSM对黑色素瘤B16细胞的细胞核形态学变化，研究OSM联合DTIC对黑色素瘤B16细胞的抑制作用；体内实验将黑色素瘤细胞B16接种于小鼠，观察OSM、DTIC及DTIC联合OSM治疗对小鼠的成瘤性的影响。结果：体外实验中OSM、DTIC或DTIC联合OSM对黑色素瘤B16细胞的增殖抑制率分别为11.2±2.3%，25.3±4.6%和32.5±3.8%，差异具有统计学意义（P<0.05），诱导黑色素瘤B16细胞的凋亡率分别为1.32±0.42%，10.64±2.13%和15.86±2.76%，差异具有统计学意义（P<0.05）。在形态学上，DTIC联合OSM可明显引起细胞核破碎增加；在体内实验中，DTIC相对于对照组能明显抑制小鼠黑色素瘤的生长，DTIC联合OSM能增加DTIC的抑瘤作用。结论：OSM联合DTIC体外可以明显抑制黑色素瘤B16细胞增殖，诱导其凋亡，为黑色素瘤的治疗提供了一种新的可能性方案。
#目的：通過體內外實驗探討抑瘤素M（OSM）聯閤達卡巴嗪（DTIC）對小鼠黑色素瘤細胞B16的抑製作用。方法：在體外分彆採用MTT法和FCM法檢測達卡巴嗪單藥以及聯閤OSM對黑色素瘤B16細胞增殖和凋亡的影響；Hochest染色法檢測達卡巴嗪單藥以及聯閤OSM對黑色素瘤B16細胞的細胞覈形態學變化，研究OSM聯閤DTIC對黑色素瘤B16細胞的抑製作用；體內實驗將黑色素瘤細胞B16接種于小鼠，觀察OSM、DTIC及DTIC聯閤OSM治療對小鼠的成瘤性的影響。結果：體外實驗中OSM、DTIC或DTIC聯閤OSM對黑色素瘤B16細胞的增殖抑製率分彆為11.2±2.3%，25.3±4.6%和32.5±3.8%，差異具有統計學意義（P<0.05），誘導黑色素瘤B16細胞的凋亡率分彆為1.32±0.42%，10.64±2.13%和15.86±2.76%，差異具有統計學意義（P<0.05）。在形態學上，DTIC聯閤OSM可明顯引起細胞覈破碎增加；在體內實驗中，DTIC相對于對照組能明顯抑製小鼠黑色素瘤的生長，DTIC聯閤OSM能增加DTIC的抑瘤作用。結論：OSM聯閤DTIC體外可以明顯抑製黑色素瘤B16細胞增殖，誘導其凋亡，為黑色素瘤的治療提供瞭一種新的可能性方案。
#목적：통과체내외실험탐토억류소M（OSM）연합체잡파진（DTIC）대소서흑색소류세포B16적억제작용。방법：재체외분별채용MTT법화FCM법검측체잡파진단약이급연합OSM대흑색소류B16세포증식화조망적영향；Hochest염색법검측체잡파진단약이급연합OSM대흑색소류B16세포적세포핵형태학변화，연구OSM연합DTIC대흑색소류B16세포적억제작용；체내실험장흑색소류세포B16접충우소서，관찰OSM、DTIC급DTIC연합OSM치료대소서적성류성적영향。결과：체외실험중OSM、DTIC혹DTIC연합OSM대흑색소류B16세포적증식억제솔분별위11.2±2.3%，25.3±4.6%화32.5±3.8%，차이구유통계학의의（P<0.05），유도흑색소류B16세포적조망솔분별위1.32±0.42%，10.64±2.13%화15.86±2.76%，차이구유통계학의의（P<0.05）。재형태학상，DTIC연합OSM가명현인기세포핵파쇄증가；재체내실험중，DTIC상대우대조조능명현억제소서흑색소류적생장，DTIC연합OSM능증가DTIC적억류작용。결론：OSM연합DTIC체외가이명현억제흑색소류B16세포증식，유도기조망，위흑색소류적치료제공료일충신적가능성방안。
Objective:To observe and identify the inhibitory effect of oncostatin M (OSM) combined with dacarbazine (DTIC) on mouse melanoma cells B16 in vitro and in vivo. Methods:The inhibitory effect of OSM combined with DTIC on the proliferation and apoptosis of B16 melanoma cell line B16 were determined through MTT assay and flow cytometry, respectively. The change in nu-cleus morphology of B16 cells was observed under a fluorescence microscope by Hoechst staining method. The effects of single agents OSM and DTIC, as well as OSM-DTIC joint treatments, on tumor in mice in vivo were observed by inoculating B16 cells into C57 BL of six mice. Results:The OSM, DTIC, and combined OSM-DTIC treatments inhibited the proliferation of B16 cells by (11.2±2.3)%, (25.3±4.6)%, and (32.5±3.8)%, respectively (P<0.05). Apoptosis of B16 occurred at (1.32±0.42)%, (10.64±2.13)%, and (15.86±2.76)%, respectively (P<0.05). Cell morphology showed a significant increase in nuclear fragmentation, as proven by OSM-DTIC combined treatment. In the in vivo experiment, DTIC caused an apparent inhibition on the growth of mouse melanoma compared with the control group, and the joint treatment showed that the addition of OSM enhanced the tumor suppression effect of DTIC. Conclusion: OSM combined with DTIC has a synergistic effect that inhibits proliferation and apoptosis of B16 in vitro. This approach suggests a new po-tential treatment for melanoma.
