CAJ | 학술논문

目的：探讨肝癌HepG2细胞AFP基因沉默对Survivin表达的影响。方法：通过siRNA技术下调肝癌HepG2细胞中AFP的表达，采用ELISA法测定转染前后上清液AFP浓度，MTT法检测转染前后细胞增殖活性，流式细胞术分析细胞凋亡率， RT-PCR检测转染前后细胞Survivin mRNA水平。结果：转染48 h后，实验组上清液中AFP浓度显著下降，肝癌细胞生长活性下降43.1%，凋亡率增加24.3%，HepG2细胞中Survivin mRNA表达降低78.0%。对照组和空白组的上述指标均无明显变化。结论：沉默肝癌HepG2细胞AFP的表达，能有效抑制肝癌细胞生长、促进细胞凋亡，这一作用可能与细胞内Survivin mRNA水平降低有关。
목적：탐토간암HepG2세포AFP기인침묵대Survivin표체적영향。방법：통과siRNA기술하조간암HepG2세포중AFP적표체，채용ELISA법측정전염전후상청액AFP농도，MTT법검측전염전후세포증식활성，류식세포술분석세포조망솔， RT-PCR검측전염전후세포Survivin mRNA수평。결과：전염48 h후，실험조상청액중AFP농도현저하강，간암세포생장활성하강43.1%，조망솔증가24.3%，HepG2세포중Survivin mRNA표체강저78.0%。대조조화공백조적상술지표균무명현변화。결론：침묵간암HepG2세포AFP적표체，능유효억제간암세포생장、촉진세포조망，저일작용가능여세포내Survivin mRNA수평강저유관。
Objective:To observe the effects of AFP gene silencing by siRNA on the Survivin mRNA of hepatocellular carcino-ma cell line HepG2. Methods:AFP gene expression was downregulated in HepG2 cell by RNAi, and the AFP content in the superna-tant was detected by ELISA. Survivin mRNA level was tested by RT-PCR. MTT was applied to evaluate cell proliferation. Flow cytom-etry was employed to observe cell apoptosis. Results:At 48 h after transfection, AFP expression was almost completely inhibited, cell proliferation activity was decreased by 43.1%, cell apoptosis rate was increased by 24.3%, and the Survivin mRNA expression was re-duced to 22.0%in the experimental group. No evident changes were observed in negative control and blank groups. Conclusion:AFP gene silenced by RNAi induces growth inhibition and apoptosis promotion of hepatocellular carcinoma cell line HepG2. This gene may be associated with the suppression of Survivin mRNA.