CAJ | 학술논문

采用胶原酶和胰酶联用的酶消化法分离小鼠骨骼肌卫星细胞，应用差速贴壁法进行纯化，并利用RT-PCR和免疫荧光染色方法对分化前后细胞的标志基因进行鉴定。结果显示：分离出的肌卫星细胞生长状态良好，RT-PCR和免疫荧光染色显示肌卫星细胞Pax7和MyoD呈阳性表达，诱导分化形成肌管后，分化标志基因MyoG和MHC呈阳性表达。本研究成功从小鼠肌肉组织中分离出了肌卫星细胞，并具有很好的体外分化能力，可以为肌肉的发育分化和损伤修复研究提供良好的细胞模型。
채용효원매화이매련용적매소화법분리소서골격기위성세포，응용차속첩벽법진행순화，병이용RT-PCR화면역형광염색방법대분화전후세포적표지기인진행감정。결과현시：분리출적기위성세포생장상태량호，RT-PCR화면역형광염색현시기위성세포Pax7화MyoD정양성표체，유도분화형성기관후，분화표지기인MyoG화MHC정양성표체。본연구성공종소서기육조직중분리출료기위성세포，병구유흔호적체외분화능력，가이위기육적발육분화화손상수복연구제공량호적세포모형。
In this study, mouse skeletal muscle satellite cells were isolated by collagenase and trypsinase digestion method. Then the satellite cells were purified and induced to differentiate into myotubes, and the marker genes of the cells before or after the differentiation were detected by RT-PCR and immunocytochemistry technology. The results show that the isolated muscle satellite cells were healthy, and expressed the marker genes of paired protein box(Pax7) and myogenic differentiation antigen (MyoD). After inducing to the myotubes, myogenin(MyoG) and myosin heavy chain(MHC)showed positive expression. The results indicate that mouse skeletal muscle satellite cells of high purity, which can provide cell sources for muscle development and damage repair study, are successfully isolated.