CAJ | 학술논문

目的研究不同采集方法制备的北柴胡中挥发油化学成分。方法分别采用水蒸气蒸馏法和顶空进样技术提取北柴胡的挥发性成分，对其进行气相色谱-质谱(GC-MS)分析。结果两种提取方法无论在成分还是相对含量方面均有一定差异，采用水蒸气蒸馏法可鉴定36种挥发性化学成分，顶空进样技术可鉴定31种挥发性化学成分，共有成分为4种，且相对含量有差异。结论两种样品采集方法分别提供了不同沸点北柴胡挥发物的化学信息；运用这两种方法可建立更全面的北柴胡挥发性成分GC-MS表征体系。
목적연구불동채집방법제비적북시호중휘발유화학성분。방법분별채용수증기증류법화정공진양기술제취북시호적휘발성성분，대기진행기상색보-질보(GC-MS)분석。결과량충제취방법무론재성분환시상대함량방면균유일정차이，채용수증기증류법가감정36충휘발성화학성분，정공진양기술가감정31충휘발성화학성분，공유성분위4충，차상대함량유차이。결론량충양품채집방법분별제공료불동비점북시호휘발물적화학신식；운용저량충방법가건립경전면적북시호휘발성성분GC-MS표정체계。
Objective To compare the difference of volatile components in Bupleurum chinense treated with different sample preparation methods. Methods Volatile components from Bupleurum chinense were extracted by steam distillation (SD) and headspace injection (HS) respectively and then analyzed by GC-MS. Results There was a certain difference between the volatile compounds extracted by the above two methods;36 volatile chemical compounds were identified in volatile oil obtained by SD, whereas 31 volatile components were identified in the herb treated with HS;among the detected compounds in the samples treated with different methods, there were 4 mutual compounds with different relative contents. Conclusion The above two methods combined with GC-MS can be used for the analysis of volatile compounds in Bupleurum chinense and thus be feasible to establish GC-MS representation system for it.