食品与药品
食品與藥品
식품여약품
FOOD AND DRUG
2013年
5期
326-328
,共3页
夏枯草穗%总黄酮%大孔吸附树脂
夏枯草穗%總黃酮%大孔吸附樹脂
하고초수%총황동%대공흡부수지
Prunella%flavonoids%macroporous adsorption resin
目的研究ADS系列大孔吸附树脂分离纯化夏枯草穗总黄酮的工艺条件及参数。方法以树脂对夏枯草穗总黄酮的吸附量和洗脱率为指标，筛选ADS系列大孔吸附树脂分离纯化夏枯草穗总黄酮的工艺条件。结果 ADS-8型大孔吸附树脂对夏枯草穗总黄酮有较好的吸附分离性能，该树脂分离纯化夏枯草穗总黄酮的最佳工艺条件为：上柱液pH值5.0，1.20 mg/mL夏枯草提取液以流速2.0 BV/h上柱，洗脱剂乙醇体积分数40%，以1.5 BV/h的流速洗脱，收集洗脱液。经上述工艺纯化后，产品总黄酮含量达82.7%，收率66.2%。结论 ADS-8型大孔吸附树脂适于分离纯化夏枯草穗总黄酮。
目的研究ADS繫列大孔吸附樹脂分離純化夏枯草穗總黃酮的工藝條件及參數。方法以樹脂對夏枯草穗總黃酮的吸附量和洗脫率為指標，篩選ADS繫列大孔吸附樹脂分離純化夏枯草穗總黃酮的工藝條件。結果 ADS-8型大孔吸附樹脂對夏枯草穗總黃酮有較好的吸附分離性能，該樹脂分離純化夏枯草穗總黃酮的最佳工藝條件為：上柱液pH值5.0，1.20 mg/mL夏枯草提取液以流速2.0 BV/h上柱，洗脫劑乙醇體積分數40%，以1.5 BV/h的流速洗脫，收集洗脫液。經上述工藝純化後，產品總黃酮含量達82.7%，收率66.2%。結論 ADS-8型大孔吸附樹脂適于分離純化夏枯草穗總黃酮。
목적연구ADS계렬대공흡부수지분리순화하고초수총황동적공예조건급삼수。방법이수지대하고초수총황동적흡부량화세탈솔위지표，사선ADS계렬대공흡부수지분리순화하고초수총황동적공예조건。결과 ADS-8형대공흡부수지대하고초수총황동유교호적흡부분리성능，해수지분리순화하고초수총황동적최가공예조건위：상주액pH치5.0，1.20 mg/mL하고초제취액이류속2.0 BV/h상주，세탈제을순체적분수40%，이1.5 BV/h적류속세탈，수집세탈액。경상술공예순화후，산품총황동함량체82.7%，수솔66.2%。결론 ADS-8형대공흡부수지괄우분리순화하고초수총황동。
Objective To investigate the technical parameters of purification process of total flavonoids from Prunella with ADS type of macroporous resin. Methods The conditions of purification of total flavonoids from Prunella were screened with adsorption capacity and desorption rate of total flavonoids as indicators. Results ADS-8 was the best resin for separation and purification of flavonoids from Prunella. The optimum conditions were:1.20 mg/mL extract of Prunella at the pH value of 5.0 with a flow rate of 2.0 BV/h to the column, the eluent was 40%ethanol, and the flow rate was 1.5 BV/h. After purified by ADS-8, the product purity was 82.7%with a yield of 66.2%. Conclusion ADS-8 can be used in purification of total flavonoids of Prunella.
