CAJ | 학술논문

背景：目前发现的促进血管增生活性最强的是血管内皮生长因子，其在类风湿关节炎滑膜细胞呈现强表达。 　　目的：观察对胶原诱导性关节炎大鼠不同时间点滑膜血管内皮生长因子表达和微血管计数的变化，探讨血管内皮生长因子和微血管在类风湿关节炎发病机制中的作用及其相关性。 　　方法：采用Ⅱ型胶原与完全福氏佐剂诱导 SD 大鼠实验性关节炎模型，采用免疫组织化学方法观察实验性关节炎大鼠不同时间点滑膜组织血管内皮生长因子的表达水平和新生血管计数，并同时观察发病时间与关节炎指数积分、血管内皮生长因子及滑膜新生血管数之间的关系。用直线相关分析法分析血管内皮生长因子表达与滑膜新生血管数之间的关系，用等级相关分析法分析关节炎指数积分与血管内皮生长因子表达及滑膜新生血管数之间的关系。 　　结果与结论：随着实验性关节炎发病时间的延长,滑膜新生血管逐渐增多，滑膜增厚，关节炎指数积分逐渐增加，血管内皮生长因子表达水平和微血管数也随之升高；其关节炎指数积分与血管内皮生长因子表达水平呈正相关关系(r=0.535、P <0.05)，血管内皮生长因子表达水平与微血管计数呈显著正相关关系(r=0.860， P<0.01)。血管内皮生长因子参与了实验性关节炎大鼠滑膜血管翳的形成过程，血管内皮生长因子与微血管密度在类风湿关节炎的发病中具有重要意义。
배경：목전발현적촉진혈관증생활성최강적시혈관내피생장인자，기재류풍습관절염활막세포정현강표체。 　　목적：관찰대효원유도성관절염대서불동시간점활막혈관내피생장인자표체화미혈관계수적변화，탐토혈관내피생장인자화미혈관재류풍습관절염발병궤제중적작용급기상관성。 　　방법：채용Ⅱ형효원여완전복씨좌제유도 SD 대서실험성관절염모형，채용면역조직화학방법관찰실험성관절염대서불동시간점활막조직혈관내피생장인자적표체수평화신생혈관계수，병동시관찰발병시간여관절염지수적분、혈관내피생장인자급활막신생혈관수지간적관계。용직선상관분석법분석혈관내피생장인자표체여활막신생혈관수지간적관계，용등급상관분석법분석관절염지수적분여혈관내피생장인자표체급활막신생혈관수지간적관계。 　　결과여결론：수착실험성관절염발병시간적연장,활막신생혈관축점증다，활막증후，관절염지수적분축점증가，혈관내피생장인자표체수평화미혈관수야수지승고；기관절염지수적분여혈관내피생장인자표체수평정정상관관계(r=0.535、P <0.05)，혈관내피생장인자표체수평여미혈관계수정현저정상관관계(r=0.860， P<0.01)。혈관내피생장인자삼여료실험성관절염대서활막혈관예적형성과정，혈관내피생장인자여미혈관밀도재류풍습관절염적발병중구유중요의의。
BACKGROUND:Vascular endothelial growth factor is found the strongest to promote angiogenesis, which show strong expression in synovial cells in rheumatoid arthritis. OBJECTIVE:To evaluate the relation between the expression of vascular endothelial growth factor and microvessel density in rheumatoid arthritis. METHODS:Col agen II and complete Freund’s adjuvant were used to induce experimental arthritis in Sprague-Dawley rats. Synovium expression of vascular endothelium growth factor and microvessel density were assayed at different time points using immunohistochemistry method, and changes in new vessels, vascular endothelial growth factor expression and arthritis index were observed along the time course. The correlations between vascular endothelial growth factor expression and number of new blood vessels as wel as between arthritis index and vascular endothelial growth factor expression were studied with linear correlation analysis and rank correlation analysis, respectively. RESULTS AND CONCLUSION:With the time lasting, new blood vessels in the synovium proliferated, the synovium was thickened, and the arthritis index rose gradual y as wel as vascular endothelial growth factor expression and number of new microvessels were on the rise. Vascular endothelial growth factor expression was positively correlated with arthritis index (r=0.535, P<0.05) and significantly positively correlated with number of new microvessels (r=0.860, P<0.01). Vascular endothelial growth factor is involved in the course of synovium pannus response, and vascular endothelial growth factor and microvessel density may play an important role in rheumatoid arthritis pathogenesis.