CAJ | 학술논문

背景：间充质干细胞移植可通过多种调节机制促进皮肤创伤修复，抑制瘢痕形成，目前多数学者认为间充质干细胞分泌的生物活性因子起主要作用。 　　目的：观察骨髓间充质干细胞条件培养液对增生性瘢痕成纤维细胞增殖能力及胶原合成的影响。 　　方法：分离培养人骨髓间充质干细胞和增生性瘢痕成纤维细胞，制备骨髓间充质干细胞条件培养液。将12，24，48 h收集的条件培养液孵育体外培养的增生性瘢痕成纤维细胞24 h，并与空白对照组比较，采用CCK-8实验检测增生性瘢痕成纤维细胞的增殖活性，Real-time PCR 检测增生性瘢痕成纤维细胞中Ⅰ型和Ⅲ型胶原的表达。 　　结果与结论：与空白对照组相比较，在24 h和48 h收集的骨髓间充质干细胞条件培养液对增生性瘢痕成纤维细胞的增殖具有明显的抑制作用(P<0.01)，对其胶原的合成也具有显著的抑制作用(P<0.01)。结果表明骨髓间充质干细胞条件培养液可通过分泌抗纤维化的生物活性因子抑制增生性瘢痕成纤维细胞的增殖和胶原产生，为细胞疗法策略减轻瘢痕提供了新的理论支持。
배경：간충질간세포이식가통과다충조절궤제촉진피부창상수복，억제반흔형성，목전다수학자인위간충질간세포분비적생물활성인자기주요작용。 　　목적：관찰골수간충질간세포조건배양액대증생성반흔성섬유세포증식능력급효원합성적영향。 　　방법：분리배양인골수간충질간세포화증생성반흔성섬유세포，제비골수간충질간세포조건배양액。장12，24，48 h수집적조건배양액부육체외배양적증생성반흔성섬유세포24 h，병여공백대조조비교，채용CCK-8실험검측증생성반흔성섬유세포적증식활성，Real-time PCR 검측증생성반흔성섬유세포중Ⅰ형화Ⅲ형효원적표체。 　　결과여결론：여공백대조조상비교，재24 h화48 h수집적골수간충질간세포조건배양액대증생성반흔성섬유세포적증식구유명현적억제작용(P<0.01)，대기효원적합성야구유현저적억제작용(P<0.01)。결과표명골수간충질간세포조건배양액가통과분비항섬유화적생물활성인자억제증생성반흔성섬유세포적증식화효원산생，위세포요법책략감경반흔제공료신적이론지지。
BACKGROUND:Mesenchymal stem celltransplantation promoted skin repair in trauma via various regulatory mechanisms and inhibited scar formation. At present, many scholars believed that bioactive factors secreted by mesenchymal stem cells played an important role. OBJECTIVE:To investigate the effects of bone marrow mesenchymal stem cellconditioned medium on the proliferation and col agen synthesis of hypertrophic scar fibroblasts. METHODS:Human bone marrow mesenchymal stem cells and hypertrophic scar fibroblasts were isolated and cultured, and bone marrow mesenchymal stem cellconditioned medium was prepared. Hypertrophic scar fibroblasts were cultured in vitro with 12, 24, and 48 hour-col ected conditioned medium for 24 hours, which was compared with blank control group. The proliferation of cells was determined by CCK-8. Type I and type III col agen expression in hypertrophic scar fibroblasts was detected using real-time PCR. RESULTS AND CONCLUSION:Compared with the blank control group, 24 and 48 hour-col ected conditioned medium significantly inhibited the proliferation of hypertrophic scar fibroblasts (P<0.01), and also suppressed col agen synthesis of hypertrophic scar fibroblasts (P<0.01). Results suggested that bone marrow mesenchymal stem cellconditioned medium inhibited the proliferation and col agen synthesis of hypertrophic scar fibroblasts by secreting anti-fibrotic bioactive factors, which may provide new theoretical supports for celltherapy to reduce cutaneous scarring.