CAJ | 학술논문

背景：COX-2基因实际存在于关节成纤维样滑膜细胞里，影响骨关节炎症的发生、发展。阐明不同分期骨关节炎滑膜细胞中COX-2基因表达量的差异对进一步深入了解骨关节炎的发生与发展，以及滑膜细胞在这一过程中的作用，具有重要的理论意义。 　　目的：分析COX-2 mRNA在不同分期骨关节炎成纤维样滑膜细胞中表达的差异。 　　方法：收集膝骨关节炎病变滑膜44例及正常滑膜12例，经原代细胞培养生长至4代的成纤维样滑膜细胞用于实验，应用实时荧光定量RT-PCR检测骨关节炎和正常成纤维样滑膜细胞中COX-2 mRNA的表达情况，最后使用2-ΔΔCt方法进行相对定量分析。 　　结果与结论：COX-2 mRNA 在骨关节炎的成纤维样滑膜细胞中的表达明显高于正常成纤维样滑膜细胞(P <0.05)，其中骨关节炎早期组表达最高，与骨关节炎中期组、晚期组比较差异有显著性意义(P <0.05)，骨关节炎中期组与晚期组中表达量差异无显著性意义(P >0.05)。结果说明COX-2mRNA可能是骨关节炎症病变过程中的重要生物学指标，且主要起作用于骨关节炎病变早期。
배경：COX-2기인실제존재우관절성섬유양활막세포리，영향골관절염증적발생、발전。천명불동분기골관절염활막세포중COX-2기인표체량적차이대진일보심입료해골관절염적발생여발전，이급활막세포재저일과정중적작용，구유중요적이론의의。 　　목적：분석COX-2 mRNA재불동분기골관절염성섬유양활막세포중표체적차이。 　　방법：수집슬골관절염병변활막44례급정상활막12례，경원대세포배양생장지4대적성섬유양활막세포용우실험，응용실시형광정량RT-PCR검측골관절염화정상성섬유양활막세포중COX-2 mRNA적표체정황，최후사용2-ΔΔCt방법진행상대정량분석。 　　결과여결론：COX-2 mRNA 재골관절염적성섬유양활막세포중적표체명현고우정상성섬유양활막세포(P <0.05)，기중골관절염조기조표체최고，여골관절염중기조、만기조비교차이유현저성의의(P <0.05)，골관절염중기조여만기조중표체량차이무현저성의의(P >0.05)。결과설명COX-2mRNA가능시골관절염증병변과정중적중요생물학지표，차주요기작용우골관절염병변조기。
BACKGROUND:COX-2 gene actual y exists in the joint fibroblast-like synoviocytes, it affects osteoarthritis occurrence and development. Understanding the differences of COX-2 gene expression levels at different stages of osteoarthritis synoviocytes has important theoretical significance for the occurrence and development of osteoarthritis, as wel as the role of synoviocytes in this process. OBJECTIVE:To analyze the difference of COX-2 mRNA at different stages of osteoarthritis fibroblast-like synoviocytes. METHODS:Synovial membrane from 44 osteoarthritis patients and 12 normal cases were selected. Primary cells were cultured to passage 4 fibroblast-like synoviocytes for the use in the experiment. COX-2 mRNA expression in osteoarthritis fibroblast-like synoviocytes and normal fibroblast-like synoviocytes was detected using real-time fluorescence quantitative RT-PCR. The relative quantitative analysis was performed using 2-ΔΔCt method. RESULTS AND CONCLUSION:Expression of COX-2 mRNA in osteoarthritis fibroblast-like synoviocytes was significantly higher than that in normal fibroblast-like synoviocytes (P<0.05). The expression levels reached a peak at early osteoarthritis group, with significant differences compared with middle and late osteoarthritis groups (P<0.05). There was no significant difference between middle and later osteoarthritis groups (P>0.05). COX-2 mRNA might be important biological marker for the inflammation in osteoarthritis, and mainly plays a role in early osteoarthritis stage.