国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2014年
9期
1221-1224
,共4页
精子%优选%凋亡%线粒体膜电位%活性氧%单细胞凝胶电泳
精子%優選%凋亡%線粒體膜電位%活性氧%單細胞凝膠電泳
정자%우선%조망%선립체막전위%활성양%단세포응효전영
Spermatozoa%Apoptosis%Mitochondrial membrane potential%Reactive oxygen specials%Single cell gel electrophoresis
目的 观察密度梯度离心法联合上游法优选精子前后各项参数的变化情况,证实优选技术对精液参数的改善作用,探讨它们之间的内在联系.方法 密度梯度离心法联合上游法精子优选前后,使用计算机辅助精子活动分析仪检测精子活动力变化;使用流式细胞仪,检测早期凋亡、MMP和细胞内ROS;使用单细胞凝胶电泳观察nDNA完整性变化.结果 优选后精子运动能力、正常形态率和nDNA完整性明显提高,差异具有统计学意义;优选前,MMP与a级精子百分比、VSL具有正相关(相关系数分别为0.641,0.708,P＜0.05);当白细胞≤1 × 106/ml时,优选前和优选后正常形态率与ROS呈负相关(相关系数分别为-0.629,-0.685,P＜0.05).结论 密度梯度离心法联合上游法优选精子是安全有效的方法;MMP能反映精子的运动能力;形态不正常的精子是ROS的重要来源.
目的 觀察密度梯度離心法聯閤上遊法優選精子前後各項參數的變化情況,證實優選技術對精液參數的改善作用,探討它們之間的內在聯繫.方法 密度梯度離心法聯閤上遊法精子優選前後,使用計算機輔助精子活動分析儀檢測精子活動力變化;使用流式細胞儀,檢測早期凋亡、MMP和細胞內ROS;使用單細胞凝膠電泳觀察nDNA完整性變化.結果 優選後精子運動能力、正常形態率和nDNA完整性明顯提高,差異具有統計學意義;優選前,MMP與a級精子百分比、VSL具有正相關(相關繫數分彆為0.641,0.708,P＜0.05);噹白細胞≤1 × 106/ml時,優選前和優選後正常形態率與ROS呈負相關(相關繫數分彆為-0.629,-0.685,P＜0.05).結論 密度梯度離心法聯閤上遊法優選精子是安全有效的方法;MMP能反映精子的運動能力;形態不正常的精子是ROS的重要來源.
목적 관찰밀도제도리심법연합상유법우선정자전후각항삼수적변화정황,증실우선기술대정액삼수적개선작용,탐토타문지간적내재련계.방법 밀도제도리심법연합상유법정자우선전후,사용계산궤보조정자활동분석의검측정자활동력변화;사용류식세포의,검측조기조망、MMP화세포내ROS;사용단세포응효전영관찰nDNA완정성변화.결과 우선후정자운동능력、정상형태솔화nDNA완정성명현제고,차이구유통계학의의;우선전,MMP여a급정자백분비、VSL구유정상관(상관계수분별위0.641,0.708,P＜0.05);당백세포≤1 × 106/ml시,우선전화우선후정상형태솔여ROS정부상관(상관계수분별위-0.629,-0.685,P＜0.05).결론 밀도제도리심법연합상유법우선정자시안전유효적방법;MMP능반영정자적운동능력;형태불정상적정자시ROS적중요래원.
Objective To observe sperm changes before and after the preparation of the density gradient centrifugation and swim-up,to confirm the role of the preferred technology in improvement the quality of the sperm,and to explore internal relations between them.Methods Semen samples were prepared by sperm prepared technology (the density gradient centrifugation and swim-up).Then early apoptosis,mitochondrial membrane potential,reactive oxygen specials were evaluated by flow cytometry analysis.Sperm parameters were examined by computer assisted sperm analysis.The nDNA integrity was evaluated by the single cell gel electrophoresis.Results Sperm motility and the percentage morphologically normal spermatozoa improved significantly after the density gradient centrifugation and swim-up compared with raw semen.MMP were significantly correlated with the percentage a sperm and VSL (r=0.641,0.708,P ＜ 0.05).ROS had the negative correlation with the percentage morphologically normal spermatozoa in non-leucocytospermia patients (r=-0.629,-0.685,P ＜ 0.05).Conclusion The method of density gradient centrifugation and swim-up was safe and effective; MMP reflect sperm motility; morphologically unnormal spermatozoa sperm is an important source of ROS in non-leucocytospermia patients.
