CAJ | 학술논문

背景：目前临床上关于温阳益髓中药治疗膝骨关节炎对软骨基质金属蛋白酶表达影响的研究还较少有报道。 　　目的：制作兔膝骨关节炎模型观察温阳益髓中药对软骨基质金属蛋白酶表达的影响。 　　方法：健康成年新西兰大白兔96只，随机选取72只采用石膏外固定方法制作兔膝骨关节炎模型。确定造模成功后再随机分为3组，模型组不做处理；中药治疗组每日灌胃方药提取液24 mL/kg，药物对照组每日灌胃葡立胶囊(盐酸氨基葡萄糖)24 mg/kg，1次/d，至造模成功后8周。另外24只新西兰大白兔作为空白对照。 　　结果与结论：PCR 方法定量分析骨关节炎模型组软骨组织中基质金属蛋白酶1、基质金属蛋白酶3、基质金属蛋白酶13表达水平均显著高于其他3组。中药治疗组及药物对照组中基质金属蛋白酶1、基质金属蛋白酶3及基质金属蛋白酶13的表达较模型组明显降低。说明温阳益髓中药治疗兔膝骨关节炎能够有效抑制兔骨关节炎软骨基质金属蛋白酶的表达。
배경：목전림상상관우온양익수중약치료슬골관절염대연골기질금속단백매표체영향적연구환교소유보도。 　　목적：제작토슬골관절염모형관찰온양익수중약대연골기질금속단백매표체적영향。 　　방법：건강성년신서란대백토96지，수궤선취72지채용석고외고정방법제작토슬골관절염모형。학정조모성공후재수궤분위3조，모형조불주처리；중약치료조매일관위방약제취액24 mL/kg，약물대조조매일관위포립효낭(염산안기포도당)24 mg/kg，1차/d，지조모성공후8주。령외24지신서란대백토작위공백대조。 　　결과여결론：PCR 방법정량분석골관절염모형조연골조직중기질금속단백매1、기질금속단백매3、기질금속단백매13표체수평균현저고우기타3조。중약치료조급약물대조조중기질금속단백매1、기질금속단백매3급기질금속단백매13적표체교모형조명현강저。설명온양익수중약치료토슬골관절염능구유효억제토골관절염연골기질금속단백매적표체。
BACKGROUND:There are few reports concerning effects of warming the yang and benefiting the marrow for the knee osteoarthritis on the expression of matrix metal oproteinase. OBJECTIVE:To observe the effect of warming the yang and benefiting the marrow on the expression of matrix metal oproteinase in rabbit models of knee osteoarthritis. METHODS:Of 96 healthy adult New Zealand rabbits, 72 rabbits were randomly selected for making rabbit models of knee osteoarthritis using plaster external fixation. After success model establishment, the rabbits were randomly assigned to three groups. Model group was left intact. Chinese medicine group received daily intragastric administration of drug extract 24 mL/kg. Drug control group was daily intragastrical y administered Puli Capsule (glucosamine hydrochloride) 24 mg/kg, once a day, until the eighth week of success model induction. An additional 24 New Zealand rabbits served as blank controls. RESULTS AND CONCLUSION:Using quantitative PCR, matrix metal oproteinase-1, matrix metal oproteinase-3 and matrix metal oproteinase-13 expression was significantly higher in the model group than that in the other three groups. Matrix metal oproteinase-1, matrix metal oproteinase-3 and matrix metal oproteinase-13 expression was significantly lower in the Chinese medicine group and drug control group than that in the model group. These results indicated that warming the yang and benefiting the marrow for knee osteoarthritis in rabbits could effectively inhibit the expression of matrix metal oproteinase in rabbits.