CAJ | 학술논문

转瓶培养细胞作为大规模培养技术成熟前的一种细胞过渡阶段,是各种培养条件（血清浓度、细胞密度、接毒时间）从实验室放大到车间生产进行优化的一个重要步骤。对转瓶技术在细胞大规模培养生产中的地位变化及现状进行了综述,为进一步优化和改进转瓶培养技术提供参考。
전병배양세포작위대규모배양기술성숙전적일충세포과도계단,시각충배양조건（혈청농도、세포밀도、접독시간）종실험실방대도차간생산진행우화적일개중요보취。대전병기술재세포대규모배양생산중적지위변화급현상진행료종술,위진일보우화화개진전병배양기술제공삼고。
Roller bottle technology was the transition stage before large-scale cell culture technology matured.It was an important step for the optimization of various conditions from laboratory to workshop,such as serum concentration,cell density and inoculation time.The status transform of roller bottle technology in mass cell culture production was reviewedto provide reference for its improvement.