中国兽药杂志
中國獸藥雜誌
중국수약잡지
CHINESE JOURNAL OF VETERINARY DRUG
2012年
5期
34-36
,共3页
林海丹%余晓%邓国东%崔成富%彭新宇%陈志虹
林海丹%餘曉%鄧國東%崔成富%彭新宇%陳誌虹
림해단%여효%산국동%최성부%팽신우%진지홍
不确定度%中兽药散剂%利巴韦林高效液相色谱%评估
不確定度%中獸藥散劑%利巴韋林高效液相色譜%評估
불학정도%중수약산제%리파위림고효액상색보%평고
uncertainty%veterinary traditional Chinese herb medicine powders%ribavirin%HPLC%evaluation
对中兽药散剂中利巴韦林含量的高效液相检测方法的不确定度进行评估,分析了各种可能引起利巴韦林测定不确定度的因素,包括标准溶液、称样、体积、稀释量具、检测仪器、色谱峰面积、重复性等进行分析,并通过计算各不确定度分量及合成标准不确定度,得出了液相色谱法测定中兽药散剂中利巴韦林含量的不确定度结果。结果显示影响较大的因素为峰面积测量、标准溶液浓度和重复性。当利巴韦林含量为0.973 mg/g时,其扩展不确定度为±0.017 mg/g。
對中獸藥散劑中利巴韋林含量的高效液相檢測方法的不確定度進行評估,分析瞭各種可能引起利巴韋林測定不確定度的因素,包括標準溶液、稱樣、體積、稀釋量具、檢測儀器、色譜峰麵積、重複性等進行分析,併通過計算各不確定度分量及閤成標準不確定度,得齣瞭液相色譜法測定中獸藥散劑中利巴韋林含量的不確定度結果。結果顯示影響較大的因素為峰麵積測量、標準溶液濃度和重複性。噹利巴韋林含量為0.973 mg/g時,其擴展不確定度為±0.017 mg/g。
대중수약산제중리파위림함량적고효액상검측방법적불학정도진행평고,분석료각충가능인기리파위림측정불학정도적인소,포괄표준용액、칭양、체적、희석량구、검측의기、색보봉면적、중복성등진행분석,병통과계산각불학정도분량급합성표준불학정도,득출료액상색보법측정중수약산제중리파위림함량적불학정도결과。결과현시영향교대적인소위봉면적측량、표준용액농도화중복성。당리파위림함량위0.973 mg/g시,기확전불학정도위±0.017 mg/g。
The ribavirin in the veterinary traditional Chinese herb medicine powders was detected by HPLC in this paper.The sources of different parts of measurement uncertainty for determination,such as standard solution,sample weight,volume,instrument,chromatographic peak and repeatability,were analyzed.And the mathematic model was established.The different parts of uncertainty were evaluated and synthesized by selected suitable metrological methods,and the expanded uncertainty in measurement was calculated.The results indicated that measurement of peak area,the concentration of standard solution and repeatability mainly affected the measurement uncertainty.When the content of ribavirin is 0.973 mg/g,the expanded uncertainty is in range of±0.017 mg/g.
