CAJ | 학술논문

该文以污染严重的珠江广州河段为研究对象，分析了感潮河段沉积物中的理化性质（机械组成、碳氮、溶解氧）和硝化细菌数量的垂向分布，并利用当地菌种，设计溶解氧浓度梯度培养硝化细菌，探讨溶解氧对硝化细菌的影响。结果表明，沉积物中的溶解氧在0～10 cm间由0.26 mg/L降低到0.02 mg/L，这主要与粉砂为主的机械组成和底栖动物的活动有关；沉积物中的硝化细菌主要受溶解氧浓度的影响，室内培养与野外实验结果对照显示，沉积物中的硝化细菌在低溶解氧条件下处于抑制状态，并导致氨氮的积累；在溶解氧浓度低于0.03 mg/L时氨氧化细菌的数量比亚硝酸氧化细菌高。
해문이오염엄중적주강엄주하단위연구대상，분석료감조하단침적물중적이화성질（궤계조성、탄담、용해양）화초화세균수량적수향분포，병이용당지균충，설계용해양농도제도배양초화세균，탐토용해양대초화세균적영향。결과표명，침적물중적용해양재0～10 cm간유0.26 mg/L강저도0.02 mg/L，저주요여분사위주적궤계조성화저서동물적활동유관；침적물중적초화세균주요수용해양농도적영향，실내배양여야외실험결과대조현시，침적물중적초화세균재저용해양조건하처우억제상태，병도치안담적적루；재용해양농도저우0.03 mg/L시안양화세균적수량비아초산양화세균고。
The aim of this study was to investigate the effect of dissolved oxygen (DO)on nitrifying bac-terial abundance and vertical distribution in sediment of Pearl River (Guangzhou section).Vertical dis-tributions of sediment physical-chemical properties (grain size distribution,concentrations of total organic carbon,total nitrogen,nitrate,ammonium,DO)and nitrifying bacteria abundance were characterized u-sing sediment cores.The experiments were conducted to cultivate bacteria using local bacteria and differ-ent DO concentrations.The results showed that DO concentrations decreased from 0.26 to 0.02 mg· L-1 within sediment depths of 0 to 10 cm,which was attributable to the sandy sediment and perturbation of benthic macrofauna.The nitrifying bacteria were inhibited by low DO condition,resulting in ammoni-um accumulation in subsurface sediment.With DO <0.03 mg·L-1 ,the abundance of ammonia oxidi-zing bacteria was greater than that of nitrite oxidizing bacteria.