CAJ | 학술논문

将丹参酮I（含量为90%）配制成不同浓度作用于体外培养的B16细胞，对B16细胞用MTT法检测药物对细胞生长抑制率，收集并采用PI染色，观察细胞的形态，通过流式细胞术（FCM）检测细胞周期及凋亡率。结果表明：不同质量浓度的（5、4、3μg/mL）丹参酮I对B16细胞的抑制作用均有明显的剂量和时间效应；丹参酮I可以使B16细胞周期的构成发生明显的变化；丹参酮I对B16细胞的生长有明显的抑制作用，作用机制之一是使S期细胞的比例降低，促使细胞凋亡。
장단삼동I（함량위90%）배제성불동농도작용우체외배양적B16세포，대B16세포용MTT법검측약물대세포생장억제솔，수집병채용PI염색，관찰세포적형태，통과류식세포술（FCM）검측세포주기급조망솔。결과표명：불동질량농도적（5、4、3μg/mL）단삼동I대B16세포적억제작용균유명현적제량화시간효응；단삼동I가이사B16세포주기적구성발생명현적변화；단삼동I대B16세포적생장유명현적억제작용，작용궤제지일시사S기세포적비례강저，촉사세포조망。
Experiments were conducted on effect of different concentrations of Tanshinone I (90%) against in vitro cultured mouse B16 melanoma cells. Cell growth inhibition rate was determined by MTT method. Treated cells were observed, collected and stained by PI. Cell cycles and apoptosis rate were detected by flow cytometry (FCM). Results demonstrated that different concentration (5, 4, 3μg /ml) of Tanshinone I had evident inhibitory effect on B16 melanoma cells with dosage and duration, and the composition of B16 cell cycles changed.