食品与药品
食品與藥品
식품여약품
FOOD AND DRUG
2013年
5期
305-307
,共3页
韦云威%徐军庆%高先领%袁建国
韋雲威%徐軍慶%高先領%袁建國
위운위%서군경%고선령%원건국
L-精氨酸%响应面%条件优化
L-精氨痠%響應麵%條件優化
L-정안산%향응면%조건우화
L-arginine%response surface%optimization
目的通过对摇瓶发酵条件的研究，提高黄色短杆菌B-003 L-精氨酸的产量。方法在单因素实验的基础上，利用响应面法，以L-精氨酸的产量为响应值，研究L-精氨酸摇瓶发酵条件。结果 Plackett-Burman设计筛选出3个对L-精氨酸产量有显著影响的因素：葡萄糖、硫酸铵和磷酸二氢钾的浓度，通过Box-Behnken试验设计得出，葡萄糖的浓度为99.57 g/L，硫酸铵浓度为60.80 g/L，磷酸二氢钾浓度为2.18 g/L时L-精氨酸产量最高。结论在最佳培养条件下，L-精氨酸产量达24.10 g/L，与最初培养条件相比产量提高了58.55%。
目的通過對搖瓶髮酵條件的研究，提高黃色短桿菌B-003 L-精氨痠的產量。方法在單因素實驗的基礎上，利用響應麵法，以L-精氨痠的產量為響應值，研究L-精氨痠搖瓶髮酵條件。結果 Plackett-Burman設計篩選齣3箇對L-精氨痠產量有顯著影響的因素：葡萄糖、硫痠銨和燐痠二氫鉀的濃度，通過Box-Behnken試驗設計得齣，葡萄糖的濃度為99.57 g/L，硫痠銨濃度為60.80 g/L，燐痠二氫鉀濃度為2.18 g/L時L-精氨痠產量最高。結論在最佳培養條件下，L-精氨痠產量達24.10 g/L，與最初培養條件相比產量提高瞭58.55%。
목적통과대요병발효조건적연구，제고황색단간균B-003 L-정안산적산량。방법재단인소실험적기출상，이용향응면법，이L-정안산적산량위향응치，연구L-정안산요병발효조건。결과 Plackett-Burman설계사선출3개대L-정안산산량유현저영향적인소：포도당、류산안화린산이경갑적농도，통과Box-Behnken시험설계득출，포도당적농도위99.57 g/L，류산안농도위60.80 g/L，린산이경갑농도위2.18 g/L시L-정안산산량최고。결론재최가배양조건하，L-정안산산량체24.10 g/L，여최초배양조건상비산량제고료58.55%。
Objective To improve the fermentation yield of L-arginine by optimizing the fermentation conditions of Brevibacterium flavum B-003. Methods On the basis of single factor experiment, response surface methodology was exploited in the study to optimize fermentation conditions for L-arginine production. Results It was indicated that the concentration of glucose, (NH4)2SO4 and KH2PO4 had significant influences on the production of L-arginine by Plackett-Burman design. The optimal concentrations of glucose, (NH4)2SO4 and KH2PO4 were 99.57, 60.8 and 2.18 g/L, respectively, obtained by Box-Behnken design. Conclusion Under the condition, L-arginine production could reach 24.10 g/L, increased by 58.55%.
