CAJ | 학술논문

目的：探讨端粒酶逆转录酶（human telomerase reverse transcriptase，hTERT）在结直肠癌（colorectal cancer，CRC）和相应正常组织中mRNA的表达，分析hTERT mRNA与结直肠癌患者临床病理参数及预后的关系。方法以实时荧光定量PCR （quantitative real-time polymerase chain reaction，qRT-PCR）法检测42例结直肠癌组织及其相应正常组织中hTERT mRNA的表达，分析其与临床病理参数之间的关系，并结合临床随访资料分析其与结直肠癌患者预后的关系。结果 hTERT mRNA在结直肠癌组织和正常组织均可表达，但结直肠癌组织中hTERT mRNA的表达水平明显高于相应正常组织，两者差异有统计学意义（P=0.011）；在有淋巴结转移的结直肠癌中hTERT mRNA高表达率比无淋巴结转移结直肠癌明显增高（92.0% vs 52.9%，P=0.011）；在结直肠癌临床 TNM Ⅲ期/Ⅳ期中hTERT mRNA的高表达率明显高于Ⅰ期/Ⅱ期（92.3%vs 50.0%，P=0.006）。通过Kaplan-Meier分析，结直肠癌患者hTERT mRNA的低表达组和高表达组之间的生存曲线差异具有统计学意义（P=0.014），提示hTERT mRNA的高表达可能与预后不良相关。结论 hTERT不仅在结直肠癌的发生、发展中起重要作用，而且可能促进结直肠癌的浸润、转移，有望成为结直肠癌的预后标志物及治疗靶点。
목적：탐토단립매역전록매（human telomerase reverse transcriptase，hTERT）재결직장암（colorectal cancer，CRC）화상응정상조직중mRNA적표체，분석hTERT mRNA여결직장암환자림상병리삼수급예후적관계。방법이실시형광정량PCR （quantitative real-time polymerase chain reaction，qRT-PCR）법검측42례결직장암조직급기상응정상조직중hTERT mRNA적표체，분석기여림상병리삼수지간적관계，병결합림상수방자료분석기여결직장암환자예후적관계。결과 hTERT mRNA재결직장암조직화정상조직균가표체，단결직장암조직중hTERT mRNA적표체수평명현고우상응정상조직，량자차이유통계학의의（P=0.011）；재유림파결전이적결직장암중hTERT mRNA고표체솔비무림파결전이결직장암명현증고（92.0% vs 52.9%，P=0.011）；재결직장암림상 TNM Ⅲ기/Ⅳ기중hTERT mRNA적고표체솔명현고우Ⅰ기/Ⅱ기（92.3%vs 50.0%，P=0.006）。통과Kaplan-Meier분석，결직장암환자hTERT mRNA적저표체조화고표체조지간적생존곡선차이구유통계학의의（P=0.014），제시hTERT mRNA적고표체가능여예후불량상관。결론 hTERT불부재결직장암적발생、발전중기중요작용，이차가능촉진결직장암적침윤、전이，유망성위결직장암적예후표지물급치료파점。
Objective To investigate the expression profile of human telomerase reverse transcriptase(hTERT)in colorectal cancer (CRC)and to assess its clinical significance. Methods Quantitative real-time PCR was used to detect expression of hTERT mRNA both in colorectal cancer and in corresponding normal tissue. Expression data were correlated with clinicopathological features,and survival analysis was performed to assess the prognostic significance. Results The hTERT mRNA was expressed in both tumor and CRC tissue,but it was expressed at significantly higher levels in cancer tissue (P=0.011). Upregulation of hTERT was significantly associated with lymph node involvement(P=0.011)and TNM stage(P=0.006). Kaplan-Meier survival analysis of low vs. high expressers of hTERT revealed much lower survival among high expressers (P=0.014). Conclusions The results of the current study suggest that hTERT may play an important role in onset of CRC,so the protein may have potential as a prognostic biomarker or even as a therapeutic target.