中国医药指南
中國醫藥指南
중국의약지남
CHINA MEDICINE GUIDE
2013年
31期
1-2,4
,共3页
补骨脂丙酮提取物%骨质疏松%分子机制
補骨脂丙酮提取物%骨質疏鬆%分子機製
보골지병동제취물%골질소송%분자궤제
Psoralen acetone extract%Osteoporosis%Molecular mechanisms
目的：考察补骨脂丙酮提取物的抗骨质疏松作用及其机制。方法采用大鼠去卵巢骨质疏松模型，通过影像学、形态学、分子生物学方法考察补骨脂丙酮提取物的抗骨质疏松作用及其机制。结果模型组的骨密度明显下降（P＜0.05）；而H0高、中剂量组则显著提升（P＜0.05）。HE染色显示：模型组股骨骨小梁遭到严重破坏，而仙灵骨葆组、H0高、中剂量组骨小梁网状结构的完整性好。荧光定量PCR显示，模型组股骨β-ERmRNA水平与对照组没有显著差异。结论补骨脂丙酮提取物H0组分高、中剂量组初步显示抗骨质疏松特性，其机制与骨组织里β型雌激素受体无关。
目的：攷察補骨脂丙酮提取物的抗骨質疏鬆作用及其機製。方法採用大鼠去卵巢骨質疏鬆模型，通過影像學、形態學、分子生物學方法攷察補骨脂丙酮提取物的抗骨質疏鬆作用及其機製。結果模型組的骨密度明顯下降（P＜0.05）；而H0高、中劑量組則顯著提升（P＜0.05）。HE染色顯示：模型組股骨骨小樑遭到嚴重破壞，而仙靈骨葆組、H0高、中劑量組骨小樑網狀結構的完整性好。熒光定量PCR顯示，模型組股骨β-ERmRNA水平與對照組沒有顯著差異。結論補骨脂丙酮提取物H0組分高、中劑量組初步顯示抗骨質疏鬆特性，其機製與骨組織裏β型雌激素受體無關。
목적：고찰보골지병동제취물적항골질소송작용급기궤제。방법채용대서거란소골질소송모형，통과영상학、형태학、분자생물학방법고찰보골지병동제취물적항골질소송작용급기궤제。결과모형조적골밀도명현하강（P＜0.05）；이H0고、중제량조칙현저제승（P＜0.05）。HE염색현시：모형조고골골소량조도엄중파배，이선령골보조、H0고、중제량조골소량망상결구적완정성호。형광정량PCR현시，모형조고골β-ERmRNA수평여대조조몰유현저차이。결론보골지병동제취물H0조분고、중제량조초보현시항골질소송특성，기궤제여골조직리β형자격소수체무관。
Objective To study preventive effects and mechanism of Psoralen acetone extract on osteoporosis. Methods Ovariectomized rats were used as the model of osteoporosis,and preventive effects and mechanism of Psoralen acetone extract on osteoporosis were investigated by the methods of radiology, morphology, molecular biology.Result The BMD of model group decreased signiifcantly(P<0.05) , while H0 groups with high dose and middle dose were signiifcantly increased (P<0.05). HE staining showed that: femoral trabecular bone of model group was severely damaged, but that of Xianlinggubao group, H0 groups with high dose and middle dose had kept a well shape of mesh. Fluorescent quantitation PCR showed that right femur of rats of β-type estrogen receptor mRNA levels were not signiifcantly different comparing with the control group. Conclusion The Psoralen acetone extract H0 at high dose and middle dose showed effects against osteoporosis of rats. The mechanism of anti-osteoporosis of H0 component had nothing to do with the β-type estrogen receptor in osteoblasts.
