CAJ | 학술논문

目的：针对大鼠视网膜石蜡切片过程中易出现标本脱离及断裂分离的问题，寻找并优化大鼠视网膜石蜡切片的制作方法并评估效果。方法分别用10%多聚甲醛固定液；4%多聚甲醛固定液；单独固定及使用4%多聚甲醛、95%酒精、冰醋酸混合固定液（FAA固定液）；预处理后再使用10%多聚甲醛分别固定等4种方法对大鼠视网膜组织进行固定，HE染色后显微镜下进行观察。结果单独使用10%多聚甲醛以及4%多聚甲醛固定均出现视网膜脱离及各层结构断裂分离现象，但经FAA固定液预处理的视网膜切片HE染色后颜色鲜明均匀，虽部分区域视网膜偶有脱离，但不易脱片，各层结构完整，未出现断裂分离。结论经FAA固定液及多聚甲醛处理过的视网膜石蜡切片固定效果明显优于单独使用多聚甲醛，甲醛浓度对HE染色结果无明显影响。
목적：침대대서시망막석사절편과정중역출현표본탈리급단렬분리적문제，심조병우화대서시망막석사절편적제작방법병평고효과。방법분별용10%다취갑철고정액；4%다취갑철고정액；단독고정급사용4%다취갑철、95%주정、빙작산혼합고정액（FAA고정액）；예처리후재사용10%다취갑철분별고정등4충방법대대서시망막조직진행고정，HE염색후현미경하진행관찰。결과단독사용10%다취갑철이급4%다취갑철고정균출현시망막탈리급각층결구단렬분리현상，단경FAA고정액예처리적시망막절편HE염색후안색선명균균，수부분구역시망막우유탈리，단불역탈편，각층결구완정，미출현단렬분리。결론경FAA고정액급다취갑철처리과적시망막석사절편고정효과명현우우단독사용다취갑철，갑철농도대HE염색결과무명현영향。
Objective To solve the problem that the rat retina paraffin sections are easily exfoliated from the slides and each layer are easily separation and fracture,we need to find a way to improve the retina paraffin section method and evaluate the tissue fixation. Methods We used 4 fixation liquids including 10%paraformaldehyde,4%paraformaldehyde, 4% paraformaldehyde and 95% alcohol and glacial acetic acid mixed liquid (FAA fixatiue solution ) combined with paraformaldehyde to fix the retinal tissue, and observed the fixation efficacy under microscope after HE staining. Results The effects of 10%paraformaldehyde and 4%paraformaldehyde fixed samples showed moderate separation and fracture of retina,but the HE staining retinal slices pre-treated by the FAA fixafive solution had bright and uniform color,although occasionally some parts of the retina were exfoliated from the slide, but it was not easy to take off,and had complete structure without separation and rupture. Conclusion The retina paraffin section fixed by FAA ixafive solution with 4% paraformaldehyde is superior to pure paraformaldehyde, and the paraformaldehyde concentration has no obviously influence on HE staining results.