CAJ | 학술논문

目的：比较基质金属蛋白酶9（matrix metalloproteinase 9， MMP-9）表达在机械法获得破骨细胞（osteoclasts，OC）和诱导法获得破骨样细胞（osteoclast like cells，OLC）的差异，为体外培养OC提供实验依据．方法采用机械分离方法，从1 d龄SD大鼠四肢长骨获得成熟OC；利用RANKL （100 ng/mL）和M-CSF （100 ng/mL）诱导RAW264.7细胞形成 OLC．采用免疫细胞化学和原位杂交技术检测不同方法获得的 OC 和 OLC MMP-9蛋白及其mRNA的表达．结果诱导法12 d组的积分光密度和平均光密度均明显高于机械法3 d组．结论诱导12 d的OLC MMP-9及其mRNA表达的明显高于机械法3 d，为OLC提供了进一步的实验数据．
목적：비교기질금속단백매9（matrix metalloproteinase 9， MMP-9）표체재궤계법획득파골세포（osteoclasts，OC）화유도법획득파골양세포（osteoclast like cells，OLC）적차이，위체외배양OC제공실험의거．방법채용궤계분리방법，종1 d령SD대서사지장골획득성숙OC；이용RANKL （100 ng/mL）화M-CSF （100 ng/mL）유도RAW264.7세포형성 OLC．채용면역세포화학화원위잡교기술검측불동방법획득적 OC 화 OLC MMP-9단백급기mRNA적표체．결과유도법12 d조적적분광밀도화평균광밀도균명현고우궤계법3 d조．결론유도12 d적OLC MMP-9급기mRNA표체적명현고우궤계법3 d，위OLC제공료진일보적실험수거．
Objective To investigate the differences of protein and mRNA expression of matrix metalloproteinase 9 (MMP-9) in osteoclasts (OC) and osteoclast-like cells (OLC) obtained by mechanical and inducement methods. Methods Mechanical separation method was used to separate mature osteoclasts from long bones of SD rats aged one-day;and inducement culture method was applied to induce OLC by using RANKL (100 ng/mL) and M-CSF (100 ng/mL) . The protein expression of MMP-9 was measured by immunocytochemistry and mRNA expression of MMP-9 was assayed by in situ hybridization. Results The integral optical density (IOD) and average optical density (AOD) of positive cells in the visual field were higher in the 12-d group of inducement method as compared with the 3 d-group of mechanical method. Conclusions It is suggested that the protein and mRNA expression of MMP-9 in OLC obtained by 12 d inducement method is much high than in OC obtained by 3 d mechanical method. OLC obtained by inducement method can be applied in the study of osteoporosis.