CAJ | 학술논문

目的：新型力学加载式生物反应器相同振动频率和不同振动应变作用于体外三维支架条件下培养的雄性大鼠成骨细胞，探究OPG/RANKL表达的影响。方法雄性大鼠成骨细胞培养、传代后分组：振动频率为1.0 Hz ，不同振动应变（A B C三组）及对照组（无振动应变及振动频率）（D组）4组。应用 RT-PCR和Western blot检测干预后OPG和RANKL mRNA和蛋白水平的表达。结果仅振动应变2000με（B组）作用于细胞后，细胞中 OPGmRNA 蛋白水平表达升高，RANKLmRNA 及蛋白水平则降低（均P＜0.05）。其余实验组未见阳性反应。结论通过新型力学加载式生物反应器（振动应变2000με振动频率1.0 Hz）的压应力作用于细胞后可以正性调节OPG/RANKL轴的表达。
목적：신형역학가재식생물반응기상동진동빈솔화불동진동응변작용우체외삼유지가조건하배양적웅성대서성골세포，탐구OPG/RANKL표체적영향。방법웅성대서성골세포배양、전대후분조：진동빈솔위1.0 Hz ，불동진동응변（A B C삼조）급대조조（무진동응변급진동빈솔）（D조）4조。응용 RT-PCR화Western blot검측간예후OPG화RANKL mRNA화단백수평적표체。결과부진동응변2000με（B조）작용우세포후，세포중 OPGmRNA 단백수평표체승고，RANKLmRNA 급단백수평칙강저（균P＜0.05）。기여실험조미견양성반응。결론통과신형역학가재식생물반응기（진동응변2000με진동빈솔1.0 Hz）적압응력작용우세포후가이정성조절OPG/RANKL축적표체。
Objective to model the same vibration frequency of the mechanical loading bioreactor and different vibration strain effect on the three-dimensional scaffold in vitro cultured osteoblast in male rats ,influence on OPG/RANKL expression .Methods the male rat osteoblast culture ,subculture group :vibration frequency is 1 Hz ,the different vibration strain (ABC three group) and control group (no vibration strain and vibration frequency) (group D) of 4 groups .Expression of OPG and RANKL mRNA and protein levels of RT-PCR and Western blot detection after intervention .Results only vibration strain 2000 με (group B) acting on cells ,elevated levels of OPGmRNA protein expression in RANKLmRNA cells ,and the protein level decreased (all P<0.05) .The experimental group had no positive reac-tion .Conclusion by the new type of mechanical loading bioreactor (vibration strain 2000 μεvibration frequency 1 Hz) pressure expression for the stress acting on cells can positively regulate the OPG/RANKL axis .