中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
8期
1205-1210
,共6页
徐凤娥%赵兵新%孙树茂%孟永春%南开辉%俞阿勇%瞿佳
徐鳳娥%趙兵新%孫樹茂%孟永春%南開輝%俞阿勇%瞿佳
서봉아%조병신%손수무%맹영춘%남개휘%유아용%구가
生物材料%纳米材料%药物控释材料%壳聚糖纳米粒%丙酮酸乙酯%反相高效液相色谱%包封率%相对标准偏差
生物材料%納米材料%藥物控釋材料%殼聚糖納米粒%丙酮痠乙酯%反相高效液相色譜%包封率%相對標準偏差
생물재료%납미재료%약물공석재료%각취당납미립%병동산을지%반상고효액상색보%포봉솔%상대표준편차
biocompatible materials%chitosan%nanoparticles%pyruvic acid
背景：有关丙酮酸乙酯含量检测方法的研究较少，采用反相高效液相色谱法检测丙酮酸乙酯含量的文献更少。目的：建立测定壳聚糖纳米粒中丙酮酸乙酯含量的反相高效液相色谱法。<br> 方法：采用Agilent1200系列高效液相色谱仪检测丙酮酸乙酯-壳聚糖纳米粒中丙酮酸乙酯的含量。色谱柱：ZORBAX Eclipse XDB-C18(4.6 mm×150 mm，5μm)，柱温25℃，流动相为乙腈-水(体积比为40∶60)，流速1 mL/min，检测波长210 nm，进样量20μL。<br> 结果与结论：丙酮酸乙酯峰与辅料及溶剂峰分离良好，丙酮酸乙酯质量浓度在1-100 mg/L内于峰面积线性关系良好(r =0.9996)，低、中、高浓度丙酮酸乙酯对照品溶液日内、日间精密度的相对标准偏差均小于3%，重复性实验的相对标准偏差为1.25%，稳定性实验的相对标准偏差为1.3%。3批样品的加样回收率分别为(91.5±1.0)%，(93.5±0.2)%，(94.4±0.4)%；包封率分别为(87.20±0.22)%，(90.50±0.15)%，(91.10±0.17)%。不同批次样品丙酮酸乙酯含量检测结果的相对标准偏差分别为0.9%，0.5%，0.3%。说明反相高效液相色谱法灵敏度高、线性范围宽、专属性强、精密度高、加样回收率好、结果精确可靠，可用于壳聚糖纳米粒中丙酮酸乙酯含量的测定。
揹景：有關丙酮痠乙酯含量檢測方法的研究較少，採用反相高效液相色譜法檢測丙酮痠乙酯含量的文獻更少。目的：建立測定殼聚糖納米粒中丙酮痠乙酯含量的反相高效液相色譜法。<br> 方法：採用Agilent1200繫列高效液相色譜儀檢測丙酮痠乙酯-殼聚糖納米粒中丙酮痠乙酯的含量。色譜柱：ZORBAX Eclipse XDB-C18(4.6 mm×150 mm，5μm)，柱溫25℃，流動相為乙腈-水(體積比為40∶60)，流速1 mL/min，檢測波長210 nm，進樣量20μL。<br> 結果與結論：丙酮痠乙酯峰與輔料及溶劑峰分離良好，丙酮痠乙酯質量濃度在1-100 mg/L內于峰麵積線性關繫良好(r =0.9996)，低、中、高濃度丙酮痠乙酯對照品溶液日內、日間精密度的相對標準偏差均小于3%，重複性實驗的相對標準偏差為1.25%，穩定性實驗的相對標準偏差為1.3%。3批樣品的加樣迴收率分彆為(91.5±1.0)%，(93.5±0.2)%，(94.4±0.4)%；包封率分彆為(87.20±0.22)%，(90.50±0.15)%，(91.10±0.17)%。不同批次樣品丙酮痠乙酯含量檢測結果的相對標準偏差分彆為0.9%，0.5%，0.3%。說明反相高效液相色譜法靈敏度高、線性範圍寬、專屬性彊、精密度高、加樣迴收率好、結果精確可靠，可用于殼聚糖納米粒中丙酮痠乙酯含量的測定。
배경：유관병동산을지함량검측방법적연구교소，채용반상고효액상색보법검측병동산을지함량적문헌경소。목적：건립측정각취당납미립중병동산을지함량적반상고효액상색보법。<br> 방법：채용Agilent1200계렬고효액상색보의검측병동산을지-각취당납미립중병동산을지적함량。색보주：ZORBAX Eclipse XDB-C18(4.6 mm×150 mm，5μm)，주온25℃，류동상위을정-수(체적비위40∶60)，류속1 mL/min，검측파장210 nm，진양량20μL。<br> 결과여결론：병동산을지봉여보료급용제봉분리량호，병동산을지질량농도재1-100 mg/L내우봉면적선성관계량호(r =0.9996)，저、중、고농도병동산을지대조품용액일내、일간정밀도적상대표준편차균소우3%，중복성실험적상대표준편차위1.25%，은정성실험적상대표준편차위1.3%。3비양품적가양회수솔분별위(91.5±1.0)%，(93.5±0.2)%，(94.4±0.4)%；포봉솔분별위(87.20±0.22)%，(90.50±0.15)%，(91.10±0.17)%。불동비차양품병동산을지함량검측결과적상대표준편차분별위0.9%，0.5%，0.3%。설명반상고효액상색보법령민도고、선성범위관、전속성강、정밀도고、가양회수솔호、결과정학가고，가용우각취당납미립중병동산을지함량적측정。
BACKGROUND:Research on ethyl pyruvate detection methods is reported rarely, and moreover, literature about reversed-phase high-performance liquid chromatography (RP-HPLC) for detection of ethyl pyruvate is less. <br> OBJECTIVE:To establish an RP-HPLC method for determination of ethyl pyruvate in ethyl pyruvate-chitosan nanoparticles. <br> METHODS: The chromatographic analysis was performed on a ZORBAX Eclipse XDB-C18 column (4.6 mm× 150 mm, 5μm) at 25℃, with the mixture of acetonitrile and water (40:60, V/V) as the mobile phase at the flow rate of 1 mL/min. The determination wavelength wasset at 210 nm and the injection volume was 20 μL. <br> RESULTS AND CONCLUSION: The peak of ethyl pyruvate and the peaks of auxiliary materials and solvent were separated wel. The linear rang of ethyl pyruvate was 1-100 mg/L (r=0.999 6). The relative standard deviation of both the intra-and inter-day precision was less than 3% for low-, moderate-, and high-concentration ethyl pyruvate. The relative standard deviation of reproducibility test and stability test was 1.25% and 1.3%, respectively. Sample average recovery rates were (91.5±1.0)%, (3.5±0.2)%, (94.4±0.4)%, respectively. Encapsulation efficiency of samples were (87.2±0.22)%, (90.5±0.15)%, (91.1±0.17)%, respectively. The relative standard deviation of different sample content were 0.9%, 0.5%, 0.3%, respectively. The RP-HPLC method for determination of ethyl pyruvate is sensitive, accurate and highly specific with wide linear range and high sample average recovery.
