中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
8期
1198-1204
,共7页
王菲%周洪%郭昱成%苏晓霞%饶国洲%赵小鹏
王菲%週洪%郭昱成%囌曉霞%饒國洲%趙小鵬
왕비%주홍%곽욱성%소효하%요국주%조소붕
生物材料%骨生物材料%纳米羟基磷灰石/壳聚糖/聚丙交酯支架%骨髓间充质干细胞%组织工程%碱性磷酸酶
生物材料%骨生物材料%納米羥基燐灰石/殼聚糖/聚丙交酯支架%骨髓間充質榦細胞%組織工程%堿性燐痠酶
생물재료%골생물재료%납미간기린회석/각취당/취병교지지가%골수간충질간세포%조직공정%감성린산매
biocompatible materials%nanoparticles%chitosan%hydroxyapatites
背景:研究发现将聚丙交酯、纳米羟基磷灰石或聚丙交酯两两复合后可在一定程度上改善支架的机械性能、生物相容性和对细胞的成骨诱导分化,但离理想的骨组织工程支架材料尚有一定的距离。<br> 目的:对比不同配比纳米羟基磷灰石/壳聚糖/聚丙交酯支架的体外生物相容性及生物活性。<br> 方法:采用粒子沥滤法制备纳米羟基磷灰石、壳聚糖、聚丙交酯的质量比分别为10∶10∶80、10∶20∶70、20∶10∶70的复合支架。将3组复合支架与人骨髓间充质干细胞进行体外复合培养,绘制细胞在支架上的生长曲线,以RT-PCR检测细胞碱性磷酸酶活性和骨钙素的表达。<br> 结果与结论:配比为20∶10∶70复合支架的细胞黏附率明显高于其他两复合支架组(P <0.01),并且复合培养9-27 d的细胞碱性磷酸酶活性、复合培养15-27 d的骨钙素表达明显高于其他两复合支架组(P <0.01),3组细胞的增殖趋势相似。说明配比为20∶10∶70的纳米羟基磷灰石/壳聚糖/聚丙交酯支架具有良好的生物相容性及骨诱导活性。
揹景:研究髮現將聚丙交酯、納米羥基燐灰石或聚丙交酯兩兩複閤後可在一定程度上改善支架的機械性能、生物相容性和對細胞的成骨誘導分化,但離理想的骨組織工程支架材料尚有一定的距離。<br> 目的:對比不同配比納米羥基燐灰石/殼聚糖/聚丙交酯支架的體外生物相容性及生物活性。<br> 方法:採用粒子瀝濾法製備納米羥基燐灰石、殼聚糖、聚丙交酯的質量比分彆為10∶10∶80、10∶20∶70、20∶10∶70的複閤支架。將3組複閤支架與人骨髓間充質榦細胞進行體外複閤培養,繪製細胞在支架上的生長麯線,以RT-PCR檢測細胞堿性燐痠酶活性和骨鈣素的錶達。<br> 結果與結論:配比為20∶10∶70複閤支架的細胞黏附率明顯高于其他兩複閤支架組(P <0.01),併且複閤培養9-27 d的細胞堿性燐痠酶活性、複閤培養15-27 d的骨鈣素錶達明顯高于其他兩複閤支架組(P <0.01),3組細胞的增殖趨勢相似。說明配比為20∶10∶70的納米羥基燐灰石/殼聚糖/聚丙交酯支架具有良好的生物相容性及骨誘導活性。
배경:연구발현장취병교지、납미간기린회석혹취병교지량량복합후가재일정정도상개선지가적궤계성능、생물상용성화대세포적성골유도분화,단리이상적골조직공정지가재료상유일정적거리。<br> 목적:대비불동배비납미간기린회석/각취당/취병교지지가적체외생물상용성급생물활성。<br> 방법:채용입자력려법제비납미간기린회석、각취당、취병교지적질량비분별위10∶10∶80、10∶20∶70、20∶10∶70적복합지가。장3조복합지가여인골수간충질간세포진행체외복합배양,회제세포재지가상적생장곡선,이RT-PCR검측세포감성린산매활성화골개소적표체。<br> 결과여결론:배비위20∶10∶70복합지가적세포점부솔명현고우기타량복합지가조(P <0.01),병차복합배양9-27 d적세포감성린산매활성、복합배양15-27 d적골개소표체명현고우기타량복합지가조(P <0.01),3조세포적증식추세상사。설명배비위20∶10∶70적납미간기린회석/각취당/취병교지지가구유량호적생물상용성급골유도활성。
BACKGROUND:Studies have found that combination of two of chitosan (CS), nano-hydroxyapatite (nHA) and poly(lactide-co-glycolide) (PLGA) can improve the mechanical properties and biocompatibility of the composite stent in certain extent as wel as improve osteogenic differentiation of the cels, but there is a certain distance from the ideal bone tissue engineering scaffolds. <br> OBJECTIVE:To study biocompatibility and osteoinductive activity of nHA/CS/PLGA scaffolds with different proportions in vitro. <br> METHODS: nHA/CS/PLGA scaffolds were prepared at mass ratio of 10:10:80, 10:20:70, 20:10:70 respectively by particle leaching method. And human bone marrow stem cels (hBMSCs) were co-cultured with these scaffolds in vitro. Adhesion, proliferation, and osteoinductive activity of these scaffolds were examined qualitatively and quantitatively by growth curve of hBMSCs on scaffolds. Gene expression of alkaline phosphatase activity and osteocalcin was detected by RT-PCR. <br> RESULTS AND CONCLUSION: hBMSCs could be attached, proliferated, and osteoinduced better on the nHA/CS/PLGA scaffold with the mass ratio of 20:10:70, compared to the other two groups of scaffolds. The differences were significant statisticaly (P< 0.05). Alkaline phosphatase and osteocalcin expressions were respectively higher in the scaffold with the mass ratio of 20:10:70 after 9-27 days of co-culture and 15-27 days of co-culture, in comparison with the other two groups of scaffolds. These findings indicate that the nHA/CS/PLGA scaffolds with the mass ratio of 20:10:70 demonstrated preferable biocompatibility and osteogenic inductivity, which is expected to be a promising scaffold material for bone tissue engineering.