CAJ | 학술논문

白僵菌是桑天牛的生物防治菌种之一。为探究白僵菌菌种退化的遗传机理，以便利用生物工程技术控制菌种退化、提高其生物防治效率，采用酯酶同工酶技术分析各代白僵菌株在蛋白质水平的变异情况，结果表明：球孢白僵菌酯酶同工酶酶谱共出现2～7条酶带，随着传代次数的增加，其主要酶带一直存在且酶谱类型未变，但酶的活性在不断减弱，尤其是几条主要酶带（A、B、E、C ）的活性变化更为明显；从聚类图中各菌株排列次序来看，各代菌株按照传代顺序排列，与第1代菌株比较，继代次数越多其间的类间距越大，变异越大，相似度越低，表现出一定的遗传多样性，严重影响其杀虫效果，说明酯酶同工酶技术可以在一定程度上反映白僵菌菌株的退化。
백강균시상천우적생물방치균충지일。위탐구백강균균충퇴화적유전궤리，이편이용생물공정기술공제균충퇴화、제고기생물방치효솔，채용지매동공매기술분석각대백강균주재단백질수평적변이정황，결과표명：구포백강균지매동공매매보공출현2～7조매대，수착전대차수적증가，기주요매대일직존재차매보류형미변，단매적활성재불단감약，우기시궤조주요매대（A、B、E、C ）적활성변화경위명현；종취류도중각균주배렬차서래간，각대균주안조전대순서배렬，여제1대균주비교，계대차수월다기간적류간거월대，변이월대，상사도월저，표현출일정적유전다양성，엄중영향기살충효과，설명지매동공매기술가이재일정정도상반영백강균균주적퇴화。
Beauveria bassiana was an important biocontrol strain .The study about the genetic mechanism of degeneration would contribute to the strain degradation control using biological engineering technology and the enhancement of biocontrol efficiency .In this study ,the vari-ation at the protein level of B .bassiana strains was alalyzed using esterase isozyme technolo-gy .The results showed that there are 2~7 isozyme belts ,and the main esterase isozyme belt also existes .Different subcultures show a same type of zymogram ,but its activity decreases obviously from generation to generation .Especially the activity change of a few main en-zymes is more apparent .The figure of clustering analysis shows that the generation ordering by passage .Compared with the 1st generation ,the genetic distance become longer from gen-eration to generation .In all ,more generation means more aberrance ,indicating that RAPD technology can reflect the strain degradation to some extent .beltwidth.