中国医药指南
中國醫藥指南
중국의약지남
CHINA MEDICINE GUIDE
2013年
17期
56-57
,共2页
蛇百子%齐墩果酸%HPLC
蛇百子%齊墩果痠%HPLC
사백자%제돈과산%HPLC
Hyptis suaveolents%Oleanolic acid%HPLC
目的对民间中草药假藿香不同入药部位中齐墩果酸的含量进行进行比较，以确定适宜的入药部位。方法采用RP-HPLC法测定。色谱柱：ODS-C18色谱柱（4.6mm×250mm），流动相：甲醇-0.5%磷酸水（90∶10）；流速：1.0mL/min；柱温：25℃；检测波长：210nm。结果其根、茎、叶中含量分别为0.02106%，0.02681%，0.03206%。结论叶中含量比根中高0.011%，比茎中高0.00525%，茎和根含量相近，在药材重量上茎和根占85%以上，故建议以叶入药为佳。该方法快速、准确、灵敏度高、重现性好。
目的對民間中草藥假藿香不同入藥部位中齊墩果痠的含量進行進行比較，以確定適宜的入藥部位。方法採用RP-HPLC法測定。色譜柱：ODS-C18色譜柱（4.6mm×250mm），流動相：甲醇-0.5%燐痠水（90∶10）；流速：1.0mL/min；柱溫：25℃；檢測波長：210nm。結果其根、莖、葉中含量分彆為0.02106%，0.02681%，0.03206%。結論葉中含量比根中高0.011%，比莖中高0.00525%，莖和根含量相近，在藥材重量上莖和根佔85%以上，故建議以葉入藥為佳。該方法快速、準確、靈敏度高、重現性好。
목적대민간중초약가곽향불동입약부위중제돈과산적함량진행진행비교，이학정괄의적입약부위。방법채용RP-HPLC법측정。색보주：ODS-C18색보주（4.6mm×250mm），류동상：갑순-0.5%린산수（90∶10）；류속：1.0mL/min；주온：25℃；검측파장：210nm。결과기근、경、협중함량분별위0.02106%，0.02681%，0.03206%。결론협중함량비근중고0.011%，비경중고0.00525%，경화근함량상근，재약재중량상경화근점85%이상，고건의이협입약위가。해방법쾌속、준학、령민도고、중현성호。
Objective To determinate the content of oleanolic acid in Hyptis suaveolents by RP- HPLC, and Choose appropriate medicinal organs. Methods The oleanolic acid was determined by HPLC, Column:ODS-C18 (250×4.6mm), mobile phase:methanol-water (with 0.5%H3PO4), detection at 210nm, lfow rate:1.0mL/min, column temperature:25℃. Results The determination of oleanolic acid was 0.02106%in roots,0.02681%in stems, 0.03206%in leaves. Conclusion The content in leaf than root high 0.011%, more than 0.00525%of the stem, high content of stems and roots. Stem and root content close, in medicinal materials on stem and root weight 85%, therefore, Suggest to clinical medicinal leaves as well. The established method is quick, accurate, sensitive and well repeated.
