CAJ | 학술논문

目的：比较两种不同核酸提取方法检测血清丙型肝炎病毒核糖核酸（HCV-RNA）对临床诊断的应用价值。方法对128份 HCV 抗体阳性的患者血清，同时用两种实时荧光定量聚合酶链反应（PCR）方法检测HCV-RNA并检测丙氨酸氨基转移酶（ALT）水平，这两种方法分别是用二氧化硅微粒法提取RNA和纯化柱法提取RNA。结果128份 HCV抗体阳性的血清中二氧化硅微粒法 HCV-RNA 阳性率41．41％，纯化柱法 HCV-RNA阳性率59．38％，纯化柱法优于二氧化硅微粒法，差异有统计学意义（χ2＝8．27，P＜0．05）。49份 HCV 抗体阳性的血清中ALT异常，且ALT浓度变化与纯化柱法 HCV-RNA水平呈正相关性（r＝0．95，P＜0．05）。结论血清HCV-RNA检测对丙型肝炎诊断和病情监测均有重要的临床意义，采用纯化柱法提取RNA具有更优的临床价值。
목적：비교량충불동핵산제취방법검측혈청병형간염병독핵당핵산（HCV-RNA）대림상진단적응용개치。방법대128빈 HCV 항체양성적환자혈청，동시용량충실시형광정량취합매련반응（PCR）방법검측HCV-RNA병검측병안산안기전이매（ALT）수평，저량충방법분별시용이양화규미립법제취RNA화순화주법제취RNA。결과128빈 HCV항체양성적혈청중이양화규미립법 HCV-RNA 양성솔41．41％，순화주법 HCV-RNA양성솔59．38％，순화주법우우이양화규미립법，차이유통계학의의（χ2＝8．27，P＜0．05）。49빈 HCV 항체양성적혈청중ALT이상，차ALT농도변화여순화주법 HCV-RNA수평정정상관성（r＝0．95，P＜0．05）。결론혈청HCV-RNA검측대병형간염진단화병정감측균유중요적림상의의，채용순화주법제취RNA구유경우적림상개치。
Objective To compare the value of two different methods of nucleic acid extraction for detecting serum hepatitis C RNA (HCV-RNA ) on the clinical diagnosis .Methods Two methods of real-time fluorescence quantitative PCR were applied to detect HCV-RNA in 128 HCV antibody-positive patients ,and the alanine transarni-nase (ALT) level was tested .These two methods were silica particles RNA extraction and RNA purified by column . Results In the 128 samples of HCV antibody-positive sera ,HCV-RNA positive rate was 41 .41% by silica particles RNA extraction ,and the rate was 59 .38% by using column purification RNA .Purification column method was better than silica particles ,and there was significant difference in positive rate (χ2 =8 .27 ,P<0 .05) .In 49 samples of HCV antibody-positive sera ,the ALT level was abnormal ,and there was positive correlation between ALT level and HCV-RNA content detected by purification column (r=0 .95 ,P<0 .05) .Conclusion Detecting HCV-RNA in serum could be useful for clinical diagnose and therapy effects monitoring ,and purification column method is better .