CAJ | 학술논문

目的：研究羧基磁珠吸附乙型肝炎病毒表面抗原（HBsAg ）的性能，为磁珠在蛋白质分离纯化中的应用打下基础。方法检测羧基磁珠吸附HBsAg前后的蛋白量，判断磁珠用量、HBsAg浓度、吸附温度、吸附时间对磁珠吸附HBsAg的影响。使用10 mmol/L NaO H洗脱HBsAg ，测定洗脱后 HBsAg的洗脱率和生物活性保留率。结果羧基磁珠吸附 HBsAg的性能与磁珠用量、HBsAg浓度、吸附温度和吸附时间有密切关系。磁珠吸附浓度为524．24μg/mL的血源性 HBsAg纯品后，10 mmol/L NaO H溶液洗脱 HBsAg的洗脱率是71．02％，活性保留率为88．15％。结论带有羧基的磁珠可以吸附 HBsAg ，有望用于 HBsAg的分离回收，为磁珠在蛋白质分离纯化中的应用打下基础。
목적：연구최기자주흡부을형간염병독표면항원（HBsAg ）적성능，위자주재단백질분리순화중적응용타하기출。방법검측최기자주흡부HBsAg전후적단백량，판단자주용량、HBsAg농도、흡부온도、흡부시간대자주흡부HBsAg적영향。사용10 mmol/L NaO H세탈HBsAg ，측정세탈후 HBsAg적세탈솔화생물활성보류솔。결과최기자주흡부 HBsAg적성능여자주용량、HBsAg농도、흡부온도화흡부시간유밀절관계。자주흡부농도위524．24μg/mL적혈원성 HBsAg순품후，10 mmol/L NaO H용액세탈 HBsAg적세탈솔시71．02％，활성보류솔위88．15％。결론대유최기적자주가이흡부 HBsAg ，유망용우 HBsAg적분리회수，위자주재단백질분리순화중적응용타하기출。
Objective To lay the foundation for the application of magnetic beads in protein purification by re-searching carboxyl magnetic beads adsorption performance of HBsAg .Methods The amount of magnetic beads ,HB-sAg concentration ,adsorption temperature and the influence of adsorption time by detecting the protein amount be-fore and after adsorption were detected .Results Adsorption HBsAg capacity of magnetic beads was affected by a-mount of magnetic beads ,HBsAg concentration ,adsorption temperature and adsorption time .After magnetic beads adsorption of HBsAg whose concentration was 524 .24 μg/mL ,the adsorbed HBsAg could be eluted by 10 mmol/L NaOH ,the elution rate was 71 .02% ,and the retained biological activity was 88 .15% .Conclusion Magnetic beads with carboxyl can adsorption HBsAg ,which is expected to be used for the separation and recovery of HBsAg .It has lay the foundation for the application of magnetic beads in protein purification.