CAJ | 학술논문

目的：对呼气冷凝液（EBC ）中3种肿瘤标志物进行联合检测，研究其在肺癌诊断中的临床价值，探讨该方法在临床应用的可能性，为今后的肺癌诊断和治疗效果评估提供可靠的参考依据。方法选择2011年1月15日至2012年12月15日该院肿瘤科收治的肺癌患者30例作为肺癌组，另抽取同期健康体检者30例作为健康对照组，对肺癌组患者治疗前后和健康对照组进行 EBC及血清中癌胚抗原（CEA ）、细胞角蛋白19的可溶性片段（CYFRA21-1）、血管内皮生长因子（VEGF）水平检测，并对比分析检测结果。采用标准 EBC 收集器收集 EBC ， CEA、CYFRA21-1采用化学发光法，VEGF用酶联免疫吸附法测定。结果肺癌组患者EBC及血清中CEA、CY-FRA21-1、VEGF检测水平明显高于对照组（P＜0．05），化疗后CEA、CYFRA21-1、VEGF水平较治疗前显著降低（P＜0．05），EBC中CEA、CYFRA21-1、VEGF水平较血清中低（P＜0．05）。结论对EBC中CEA、CYFRA21-1、V EG F水平进行检测对于肺癌的诊断、病理分型和疗效判断均具有重要的参考价值，EBC检测结果与血清结果联合可实现相互补充，提高阳性率，值得关注。
목적：대호기냉응액（EBC ）중3충종류표지물진행연합검측，연구기재폐암진단중적림상개치，탐토해방법재림상응용적가능성，위금후적폐암진단화치료효과평고제공가고적삼고의거。방법선택2011년1월15일지2012년12월15일해원종류과수치적폐암환자30례작위폐암조，령추취동기건강체검자30례작위건강대조조，대폐암조환자치료전후화건강대조조진행 EBC급혈청중암배항원（CEA ）、세포각단백19적가용성편단（CYFRA21-1）、혈관내피생장인자（VEGF）수평검측，병대비분석검측결과。채용표준 EBC 수집기수집 EBC ， CEA、CYFRA21-1채용화학발광법，VEGF용매련면역흡부법측정。결과폐암조환자EBC급혈청중CEA、CY-FRA21-1、VEGF검측수평명현고우대조조（P＜0．05），화료후CEA、CYFRA21-1、VEGF수평교치료전현저강저（P＜0．05），EBC중CEA、CYFRA21-1、VEGF수평교혈청중저（P＜0．05）。결론대EBC중CEA、CYFRA21-1、V EG F수평진행검측대우폐암적진단、병리분형화료효판단균구유중요적삼고개치，EBC검측결과여혈청결과연합가실현상호보충，제고양성솔，치득관주。
Objective To combinedly detect three tumor markers in exhaled breath condensate ,analyze the clinical value on diagnosis of lung cancer ,discuss the possibility of the method applied in the clinic ,and provide a reli-able basis for the lung cancer diagnosis and treatment in the future assessment .Methods 30 cases with lung cancer from January 15 ,2011 to December 15 ,2012 in our hospital were selected as lung cancer group ,and the other 30 healthy people were selected as control group .The CEA ,CYFRA21-1 and VEGF levels in exhaled breath condensate and serum were detected in lung cancer group and control group ,and the detection results were comparatively ana-lyzed .Results The CEA ,CYFRA21-1 and VEGF levels in lung cancer group were significantly higher than that of control group(P< 0 .05) .CEA ,CYFRA21-1 and VEGF levels were significantly decreased after treatment with chemotherapy than before(P< 0 .05) .In exhaled breath condensate ,the CEA ,CYFRA21-1 and VEGF levels were low than that in serum(P<0 .05) .Conclusion Detection of CEA ,CYFRA21-1 and VEGF levels in exhaled breath condensate has important reference value in lung cancer diagnosis ,pathological typing and curative effect judgment , and it is worthy of attention .