CAJ | 학술논문

目的：优化小鼠主动脉平滑肌细胞膜蛋白提取方法，获得高纯度的Robo4膜蛋白。方法高速离心法选择性地分离提取总膜蛋白，BCA法测定总膜蛋白含量，Western-Blot检测目的蛋白条带。结果成功提取总膜蛋白，总膜蛋白浓度达1．47μg/μL ，Western-Blot检测出Robo4膜蛋白条带。结论该提取方法简单、可靠、快速，获得的膜蛋白纯度高，可用于Western-Blot后续试验。
목적：우화소서주동맥평활기세포막단백제취방법，획득고순도적Robo4막단백。방법고속리심법선택성지분리제취총막단백，BCA법측정총막단백함량，Western-Blot검측목적단백조대。결과성공제취총막단백，총막단백농도체1．47μg/μL ，Western-Blot검측출Robo4막단백조대。결론해제취방법간단、가고、쾌속，획득적막단백순도고，가용우Western-Blot후속시험。
Objective To optimize the method of extracting membrane proteins in mouse aortic smooth muscle cells and harvest high-purity Robo4 membrane proteins .Methods The total membrane proteins were selectively ex-tracted by high speed centrifuge ,the total membrane protein concentrations were measured by BCA ,and Robo4 mem-brane proteins were assessed by Western-Blot .Results The total membrane proteins were successfully extracted ,the membrane protein concentration was 1 .47μg/μL ,and target protein bands were detected by western blot successful-ly .Conclusion This extraction method was simple ,reliable ,fast ,and could harvest high purity of membrane pro-teins .It can be used for follow-up testing of western blot .