CAJ | 학술논문

　　目的通过正交试验优化胡黄连苷Ⅱ治疗大鼠脑缺血损伤的最佳剂量和时间窗。方法应用双侧颈总动脉结扎法（BCCAO）建立大鼠前脑缺血模型，按照正交试验设计分组，经腹腔注射胡黄连苷Ⅱ干预治疗，硫代巴比妥酸法测定血清和脑组织中脂质过氧化物丙二醛（MDA）的含量。黄嘌呤氧化酶法检测血清和脑组织中超氧化物歧化酶（SOD）的活性。结果胡黄连苷Ⅱ治疗脑缺血损伤的最佳效果，根据血清和脑组织MDA含量分析，均为脑缺血1.5h腹腔注射10mg/kg体质量。根据血清和脑组织SOD活性分析，均为脑缺血1.5h腹腔注射20mg/kg体质量。结论从用药剂量最小化和治疗时间窗最大化的角度综合评价，胡黄连苷Ⅱ治疗脑缺血损伤的最佳治疗时间窗和剂量为脑缺血1.5 h腹腔注射10~20mg/kg体质量。
　　목적통과정교시험우화호황련감Ⅱ치료대서뇌결혈손상적최가제량화시간창。방법응용쌍측경총동맥결찰법（BCCAO）건립대서전뇌결혈모형，안조정교시험설계분조，경복강주사호황련감Ⅱ간예치료，류대파비타산법측정혈청화뇌조직중지질과양화물병이철（MDA）적함량。황표령양화매법검측혈청화뇌조직중초양화물기화매（SOD）적활성。결과호황련감Ⅱ치료뇌결혈손상적최가효과，근거혈청화뇌조직MDA함량분석，균위뇌결혈1.5h복강주사10mg/kg체질량。근거혈청화뇌조직SOD활성분석，균위뇌결혈1.5h복강주사20mg/kg체질량。결론종용약제량최소화화치료시간창최대화적각도종합평개，호황련감Ⅱ치료뇌결혈손상적최가치료시간창화제량위뇌결혈1.5 h복강주사10~20mg/kg체질량。
Objective To optimize the therapeutic dose and time window of picrosedeⅡin cerebral ischemic injury in rats by orthogonal test.Methods The forebrain ischemia models were established by bilateral common carotid artery occlusion (BCCAO) methods.The successful models were randomly grouped according to orthogonal experimental design and treated by injecting picroside Ⅱintraperitonenally at different ischemic time with different dose.The concentration of lipid perioxide malondialdehyde (MDA) in serum and tissue of was determined by thiobarbituric acid assay.The activity of superoxide dismutase (SOD) in serum and brain tissue was determined by xanthine oxidase assay.Result The optimized composition of the therapeutic dose and time window of picrosideⅡin cerebral ischemic injury was ischemia 1.5h with 10mg/kg body weight according to the concentration of MDA in serum and brain tissue, ischemia 1.5h with 20mg/kg body weight according to the activity of SOD in serum and brain tissue. Conclusion From the principle of lowest therapeutic dose with longest tome window, the optimized composition of the therapeutic dose and time window of picrosideⅡin cerebral ischemic injury is ischemia 1.5h with 10-20mg/kg body weight.